Kurata H, Achioku T, Okuda N, Furusaki S
Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo 113, Japan.
Biotechnol Prog. 1998 Sep;14(5):797-9. doi: 10.1021/bp980065u.
We developed novel equipment that intermittently illuminates Coffea arabica cell suspensions at a second-scale interval and investigated how intermittent irradiation enhances caffeine biosynthesis by C. arabica cells. The light/dark cycles consisting of 2 s of illumination and 18 s of darkness enhanced caffeine production, reaching the same level as for continuous light. The intermittent illumination increased the production efficiency regarding light consumption by a factor of 10. Caffeine production was determined by light intensity regardless of intermittent or continuous light irradiation. We propose a new concept for designing a photobioreactor that is applicable to secondary metabolite production by plant cell culture.
我们开发了一种新型设备，该设备以秒级间隔间歇性地照射阿拉伯咖啡细胞悬浮液，并研究了间歇性照射如何增强阿拉伯咖啡细胞的咖啡因生物合成。由2秒光照和18秒黑暗组成的光/暗循环提高了咖啡因产量，达到了与连续光照相同的水平。间歇性光照将光消耗的生产效率提高了10倍。无论间歇性或连续光照，咖啡因产量均由光强决定。我们提出了一种设计光生物反应器的新概念，该光生物反应器适用于植物细胞培养的次级代谢产物生产。
