[鼻咽癌及原代培养正常鼻咽上皮细胞中7q32部分基因表达图谱的建立]

[Establishment of partial gene expression map of 7q32 in nasopharyngeal carcinoma and primary culture normal nasopharyngeal epithelial cells].

作者信息

Jiang N, Zhan F, Xie Y, Zeng Z, Zhou M, Deng L, Li G

机构信息

Cancer Research Institute, Hunan Medical University, Changsha,Hunan, 410078 P. R. China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Oct 10;15(5):267-70.

PMID:9758869

Abstract

OBJECTIVE

To establish partial gene expression map of 7q32 in nasopharyngeal carcinoma (NPC) cell line, tissues and primary culture normal nasopharyngeal epithelial cells.

METHODS

We detected the expression of 20 ESTs at 7q32 in NPC cell line HNE1,13 NPC biopsies and primary culture normal nasopharyngeal epithelial cells using differential RT PCR and Northern hybridization.

RESULTS

8 ESTs (AA188181, AA13079,N27556, AA031919, N22721, H20825, T91284, AA001936) expressed equally in both of HNE1 and primary culture normal nasopharyngeal epithelial cells; 7 ESTs (T64215, AA025822, R60014,R80002,H06688, R60192,R95096) expressed in neither of them; 3 ESTs (H19830,W72688,AA130630) overexpressed in HNE1 ; and 2 ESTs (AA070437, H90882) overexpressed in primary culture normal nasopharyngeal epithelial cells. W72688 and H19830 each overexpressed in 77%(10/13) of NPC biopsies; AA070437 down-expressed in 30.7% of NPC biopsies.

CONCLUSION

Partial gene expression map of 7q32 in nasopharyngeal carcinoma cell line ,tissues and primary culture normal nasopharyngeal epithelial cells has been established. The up-regulation of W72688, H19830 and down-regulation of AA070437 may be related to the occurrence of NPC.

摘要

目的

建立鼻咽癌（NPC）细胞系、组织及原代培养的正常鼻咽上皮细胞中7q32的部分基因表达图谱。

方法

应用差异逆转录聚合酶链反应（RT-PCR）和Northern杂交技术，检测7q32区域20个表达序列标签（ESTs）在NPC细胞系HNE1、13例NPC活检组织及原代培养的正常鼻咽上皮细胞中的表达情况。

结果

8个ESTs（AA188181、AA13079、N27556、AA031919、N22721、H20825、T91284、AA001936）在HNE1细胞系和原代培养的正常鼻咽上皮细胞中表达水平相当；7个ESTs（T64215、AA025822、R60014、R80002、H06688、R60192、R95096）在两者中均未表达；3个ESTs（H19830、W72688、AA130630）在HNE1细胞系中高表达；2个ESTs（AA070437、H90882）在原代培养的正常鼻咽上皮细胞中高表达。W72688和H19830在77%（10/13）的NPC活检组织中高表达；AA070437在30.7%的NPC活检组织中低表达。