Fu Y, Zhu P, Wang W, Liu Y, Xue H
Department of Hematology, The First Affiliated Hospital, Beijing Medical University, Beijing, 100034, P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Oct 10;15(5):271-3.
To investigate the microsatellite instability (MSI) and loss of heterozygosity (LOH) near the chromosome translocation breakpoints of various types of leukemia.
PCR amplification of 11 microsatellite loci closely linked to chromosome translocation breakpoints in 30 leukemia patients.
Twenty one in 30 cases of leukemia had MSI in from 1 to 14 microsatellite loci. Eleven in 15 cases of acute leukemia had microsatellite instability (MSI) in more than 1 locus; among them 4 cases had MSI in from 7 to 11 loci. In order of MSI presence rate were D22S315>D9S179>D16S515>D8S559>D12S89. In six cases of chronic myeloid leukemia, 4 cases had MSI in more than 3 loci. Thirteen (2/15) percent of acute leukemia patients had loss of heterozygosity (LOH); the rate was higher than that in other leukemia types.
The results of MSI and LOH in many cases indicate that there are a lot of allelic alterations in leukemia patients, but those changes have no special relationship with chromosome translocation breakpoints in various leukemia types.
研究各类白血病染色体易位断点附近的微卫星不稳定性（MSI）及杂合性缺失（LOH）情况。
对30例白血病患者中与染色体易位断点紧密连锁的11个微卫星位点进行PCR扩增。
30例白血病患者中，21例在1至14个微卫星位点存在MSI。15例急性白血病患者中，11例在超过1个位点存在微卫星不稳定性（MSI）；其中4例在7至11个位点存在MSI。按MSI出现率排序为D22S315>D9S179>D16S515>D8S559>D12S89。6例慢性髓性白血病患者中，4例在超过3个位点存在MSI。15例急性白血病患者中有13（2/15）%存在杂合性缺失（LOH）；该比率高于其他类型白血病。
MSI和LOH的结果在许多病例中表明白血病患者存在大量等位基因改变，但这些改变与各类白血病中的染色体易位断点无特殊关系。
