Wada K, Kaminuma O, Mori A, Nakata A, Ogawa K, Kikkawa H, Ikezawa K, Suko M, Okudaira H
Department of Medicine and Physical Therapy, Faculty of Medicine, University of Tokyo, Tokyo, Japan.
Int Arch Allergy Immunol. 1998 Sep;117 Suppl 1:24-7. doi: 10.1159/000053566.
We have recently demonstrated that airway eosinophilic inflammation can be transferred to unprimed mice by infusion of IL-5-producing T cell clones. In this study, we investigated the effects of dexamethasone and cyclosporin A on the airway eosinophilic inflammation in mice transferred with T cell clones. An ovalbumin-reactive T cell clone, KW29, produced IL-5 as well as IL-2 and IL-4 upon stimulation with relevant antigen. Dexamethasone and cyclosporin A dose-dependently suppressed the production of these cytokines in vitro. The number of eosinophils recovered in the bronchoalveolar lavage fluid and the airway responsiveness to acetylcholine were increased in KW29-transferred mice after antigen provocation. Both responses were dose-dependently suppressed by the administration of dexamethasone or cyclosporin A in vivo. We concluded that airway eosinophilic inflammation can be controlled by agents capable of downregulating IL-5 production in T cells.
我们最近证实，通过输注产生白细胞介素-5（IL-5）的T细胞克隆，气道嗜酸性粒细胞炎症可转移至未致敏小鼠。在本研究中，我们调查了地塞米松和环孢素A对经T细胞克隆转移的小鼠气道嗜酸性粒细胞炎症的影响。一个卵清蛋白反应性T细胞克隆KW29，在受到相关抗原刺激后可产生IL-5、IL-2和IL-4。地塞米松和环孢素A在体外可剂量依赖性地抑制这些细胞因子的产生。抗原激发后，KW29转移小鼠支气管肺泡灌洗液中回收的嗜酸性粒细胞数量以及气道对乙酰胆碱的反应性均增加。在体内给予地塞米松或环孢素A后，这两种反应均受到剂量依赖性抑制。我们得出结论，气道嗜酸性粒细胞炎症可通过能够下调T细胞中IL-5产生的药物来控制。
