Varga S M, Welsh R M
Department of Pathology, University of Massachusetts Medical Center, Worcester 01655, USA.
J Immunol. 1998 Oct 1;161(7):3215-8.
Lymphocytic choriomeningitis virus (LCMV), like many viruses, induces a profound activation and expansion of CD8+ T cells. In contrast, CD4+ T cells do not increase in total number during the acute infection. We show here that mice infected with LCMV have a low but detectable frequency (<1/300) of CD4+ T cells, as detected by IL-2 production in limiting dilution assays, to each of two class II peptides during the peak of the acute LCMV response and into long-term memory. However, during the peak of the acute CD4+ T cell response, >20% of the CD4+ T cells secreted IFN-gamma after stimulation with PMA and ionomycin, and >10% of the CD4+ T cells secreted IFN-gamma after stimulation with the LCMV peptides. Thus, these new sensitive assays reveal a heretofore unappreciated, yet profound Ag-specific CD4+ T cell response during viral infections.
淋巴细胞性脉络丛脑膜炎病毒（LCMV）与许多病毒一样，可诱导CD8 + T细胞发生显著激活和扩增。相比之下，在急性感染期间，CD4 + T细胞的总数并未增加。我们在此表明，通过有限稀释试验中白细胞介素-2的产生检测到，感染LCMV的小鼠在急性LCMV反应高峰期直至长期记忆阶段，针对两种II类肽中的每一种，都有低但可检测到的频率（<1/300）的CD4 + T细胞。然而，在急性CD4 + T细胞反应高峰期，>20%的CD4 + T细胞在用佛波酯（PMA）和离子霉素刺激后分泌γ干扰素（IFN-γ），>10%的CD4 + T细胞在用LCMV肽刺激后分泌IFN-γ。因此，这些新的灵敏检测方法揭示了病毒感染期间迄今未被认识到的、但显著的抗原特异性CD4 + T细胞反应。
