Lee Z S, Critchley J A
Department of Clinical Pharmacology, The Chinese University of Hong Kong, Prince of Wales Hospital, New Territories.
Clin Chim Acta. 1998 Aug 10;276(1):89-102. doi: 10.1016/s0009-8981(98)00105-3.
Catecholamines, dopamine and the renal kallikrein-kinin system may participate in the pathogenesis of hypertension. In the past these systems have been studied independently in isolation. Attempts to study the systems together have been hampered by incompatibility of the current urine preservatives for the two assays involved. In order to measure acid-stable catecholamines and acid-labile kallikrein enzyme together, we have established boric acid solution as a preservative by comparing the stability of urinary catecholamines stored in it with the commonly employed preservative, hydrochloric acid (HCl) as well as the stability of urinary kallikrein in untreated urine with and without boric acid at ambient temperatures for 24 and 48 h, and at -20 degrees C for 2 weeks and 1, 2 and 3 months. The stability of other common urine parameters including cortisol, electrolytes, creatinine and protein, was also investigated after storage with boric acid at ambient temperature for 24 h. Our results showed that there was a good agreement between the measurements of urinary catecholamines stored in both HCl and boric acid and that the latter did not interfere with measurements of urinary kallikrein or other common urine parameters.
儿茶酚胺、多巴胺和肾激肽释放酶-激肽系统可能参与高血压的发病机制。过去，这些系统一直是单独进行研究的。将这些系统放在一起研究的尝试受到了当前用于所涉及的两种检测方法的尿液防腐剂不相容性的阻碍。为了同时测量酸稳定的儿茶酚胺和酸不稳定的激肽释放酶，我们通过比较储存在硼酸溶液中的尿儿茶酚胺与常用防腐剂盐酸（HCl）的稳定性，以及在环境温度下24小时和48小时、-20℃下2周、1个月、2个月和3个月时，有无硼酸的未处理尿液中尿激肽释放酶的稳定性，确立了硼酸溶液作为一种防腐剂。在室温下用硼酸储存24小时后，还研究了其他常见尿液参数（包括皮质醇、电解质、肌酐和蛋白质）的稳定性。我们的结果表明，储存在HCl和硼酸中的尿儿茶酚胺测量值之间有良好的一致性，并且后者不会干扰尿激肽释放酶或其他常见尿液参数的测量。
