Wade R H, Meurer-Grob P, Metoz F, Arnal I
Instiut de Biologie Structurale (CEA & CNRS), Grenoble, France.
Eur Biophys J. 1998;27(5):446-54. doi: 10.1007/s002490050155.
We present a short overview of the current status of work on the organisation and structure of microtubules and of microtubule-motor protein complexes. At present there is great interest in obtaining structural information that can help us to understand the movement of the kinesin family of microtubule associated molecular motors. Using electron cryomicroscopy and image reconstruction methods three dimensional maps of microtubule-motor complexes have been obtained in the presence of different nucleotides. We address a number of principles involved in different aspects of this work.
我们简要概述了目前关于微管及其组织、结构以及微管-马达蛋白复合体的研究现状。目前，人们对获取有助于理解与微管相关的驱动蛋白家族分子马达运动的结构信息非常感兴趣。利用电子冷冻显微镜和图像重建方法，已在不同核苷酸存在的情况下获得了微管-马达复合体的三维图谱。我们阐述了这项工作不同方面所涉及的一些原理。
