Physical mapping and activity of ribosomal RNA genes in mussel Mytilus galloprovincialis.

In bivalve molluscs, NOR analysis was carried out by silver staining, and extensive intra- and interindividual differences in the apparent number of NORs were reported. In this work, we determine the physical mapping of 18S and 28S ribosomal genes of the mussel M. galloprovincialis by fluorescence in situ hybridization (FISH). We also apply silver staining to the same individuals in order to determine if structural changes are involved in the heteromorphism detected by this technique. Our results show that rDNA loci map on the telomeric region of the long arm of two submetacentric-subtelocentric chromosome pairs. In addition to variations in NOR expression, we found some cases of structural variations that affect the number of rDNA loci between individuals and the location of the rDNA locus between the cells of the individual. We suggest that FISH should be applied to other bivalves to assess the variation of rDNA loci and undertake more accurate interspecific comparisons.