Haring E, Hagemann S, Lankinen P, Pinsker W
Museum of Natural History, Vienna, Austria.
Hereditas. 1998;128(3):235-44. doi: 10.1111/j.1601-5223.1998.00235.x.
PCR screening with primers specific for the T-, M-, and O-type P element subfamilies was performed to investigate the interspecific distribution in 18 species and to reconstruct the phylogenetic history of the various types within the obscura species group. T-type elements occur in D. ambigua, D. tristis, D. obscura, D. subsilvestris, and D. eskoi. In the genomes of D. subobscura, D. madeirensis, and D. guanche they are present in the form of terminally truncated T-type derivatives. The wide distribution suggests that the T-type subfamily had a long evolutionary history in the obscura lineage. In contrast, the patchy occurrence of M- and O-type elements can be ascribed to four independent events of horizontal invasion of different lineages. The cladogenesis of the obscura group was investigated using a partial sequence of the Adh gene as a marker. In contrast to earlier findings, the position of D. eskoi had to be revised. D. eskoi appears as the closest relative of the D. ambigua clade, whereas D. tsukubaensis is the sister taxon of the species pair D. bifasciata/D. imaii. This result is in good accordance with the P element data, where high sequence similarity (95%) was found among the T-type elements of D. eskoi and those of D. ambigua and D. tristis.
使用针对T型、M型和O型P因子亚家族的特异性引物进行聚合酶链反应（PCR）筛选，以研究18个物种间的分布情况，并重建暗果蝇物种组内不同类型的系统发育史。T型因子存在于模糊果蝇、三刺果蝇、暗果蝇、亚林果蝇和埃氏果蝇中。在暗果蝇、马德拉果蝇和加那利果蝇的基因组中，它们以末端截短的T型衍生物形式存在。广泛的分布表明T型亚家族在暗果蝇谱系中具有悠久的进化历史。相比之下，M型和O型因子的零散出现可归因于不同谱系的四次独立水平入侵事件。使用乙醇脱氢酶（Adh）基因的部分序列作为标记，研究了暗果蝇组的分支发生情况。与早期研究结果不同，埃氏果蝇的位置必须重新修订。埃氏果蝇似乎是模糊果蝇分支的最近亲缘种，而筑波果蝇是双带果蝇/今井果蝇物种对的姐妹分类单元。这一结果与P因子数据高度吻合，在埃氏果蝇与模糊果蝇和三刺果蝇的T型因子之间发现了95%的高序列相似性。
