Craig D B, Polakowski R M, Arriaga E, Wong J C, Ahmadzadeh H, Stathakis C, Dovichi N J
Department of Chemistry, University of Alberta, Edmonton, Canada.
Electrophoresis. 1998 Sep;19(12):2175-8. doi: 10.1002/elps.1150191222.
We report a method for protein labeling, separation by capillary electrophoresis in a polymer sieving matrix, and detection by laser-induced fluorescence. Different dyes are used to label standard and sample proteins. A two-spectral channel detector resolves fluorescence from the sample and standards. Comparison of the migration time of the sample and standards permits the precise determination of molecular weight, irrespective of variations in run-to-run migration times.
我们报道了一种蛋白质标记方法，该方法通过在聚合物筛分基质中进行毛细管电泳分离，并采用激光诱导荧光检测。使用不同的染料对标准蛋白和样品蛋白进行标记。双光谱通道检测器可分辨样品和标准品的荧光。比较样品和标准品的迁移时间，可精确测定分子量，而不受每次运行迁移时间变化的影响。
