Kobayashi K, Kobayashi H, Ueda M, Honda Y
Department of Ophthalmology and Visual Science, Kyoto University Graduate School of Medicine, Japan.
Invest Ophthalmol Vis Sci. 1998 Oct;39(11):2105-10.
To investigate the expression and distribution of estrogen receptor protein and mRNA in bovine and rat retinas.
Western blot analysis with an antiestrogen receptor monoclonal antibody (mAb) was used to detect estrogen receptor protein in the bovine retina. Immunohistochemistry with an antiestrogen receptor mAb and in situ hybridization with an oligodeoxynucleotide sequence coding for estrogen receptor were applied to study the cellular distribution of estrogen receptor protein and its mRNA in male bovine retina and rat retina of both sexes.
Estrogen receptor protein was detected in bovine retina by Western blot analysis. Immunohistochemical staining with the antiestrogen receptor mAb was widespread throughout the neural retina. Specific staining showed extensive distribution localizing in the nerve fiber layer, the ganglion cell layer, the inner nuclear layer, and the outer plexiform layer. Retinal pigment epithelium and choroid were also stained with the antiestrogen receptor mAb. By in situ hybridization, the expression of estrogen receptor mRNA was predominantly observed in ganglion cell layer, the inner nuclear layer, and outer portion of the outer nuclear layer. No significant difference was found between male and female rats in the immunostaining of retinas with the antiestrogen receptor mAb.
This study provides the first evidence for the presence of estrogen receptor in bovine and rat retinas. Expression of estrogen receptor throughout the retina suggests that estrogen may have important functions in the retina.
研究雌激素受体蛋白和mRNA在牛和大鼠视网膜中的表达及分布。
采用抗雌激素受体单克隆抗体（mAb）进行蛋白质免疫印迹分析，以检测牛视网膜中的雌激素受体蛋白。应用抗雌激素受体mAb进行免疫组织化学以及用编码雌激素受体的寡脱氧核苷酸序列进行原位杂交，来研究雌激素受体蛋白及其mRNA在雄性牛视网膜和两性大鼠视网膜中的细胞分布。
通过蛋白质免疫印迹分析在牛视网膜中检测到雌激素受体蛋白。用抗雌激素受体mAb进行免疫组织化学染色在整个神经视网膜中广泛分布。特异性染色显示其广泛分布于神经纤维层、神经节细胞层、内核层和外网状层。视网膜色素上皮和脉络膜也被抗雌激素受体mAb染色。通过原位杂交，主要在神经节细胞层、内核层和外核层外部观察到雌激素受体mRNA的表达。在用抗雌激素受体mAb对大鼠视网膜进行免疫染色时，雄性和雌性大鼠之间未发现显著差异。
本研究首次提供了牛和大鼠视网膜中存在雌激素受体的证据。雌激素受体在整个视网膜中的表达表明雌激素可能在视网膜中具有重要功能。
