Landers E, González-Hevia M A, Mendoza M C
Area de Microbiología, Departamento de Biología Funcional, Universidad de Oviedo, Spain.
Int J Food Microbiol. 1998 Aug 18;43(1-2):81-90. doi: 10.1016/s0168-1605(98)00092-0.
A molecular epidemiology study of Salmonella serotype Enteritidis was carried out by ribotyping performed with a mixture of PstI and SphI (PS ribotyping), and randomly amplified polymorphic DNA (RAPD) typing conducted with the OPB17 primer. A series, including 38 food and 25 water strains, which were epidemiologically unrelated and collected in Spain over 1985-1996, was differentiated into 20 PS ribotypes [discrimination index (DI) = 0.67], RAPD types (DI = 0.28), and by combining both methods into 23 genomic groups (DI = 0.76). With ribotyping data from the strains tested in this and in a previous work, including clinical and reference strains, a similarity dendrogram was traced and the subsequent branches and groupings were correlated with RAPD types, phage types and sources of origin. At a similarity level of 55%, a major cluster (grouping five subclusters and three single branches) and two minor clusters were revealed. Results supported the fact that organisms representing, at least, 40 genomic groups are currently circulating in Spain, but that only the organisms of five groups predominate and these fall into a single subcluster or lineage. Organisms of these five groups could be considered endemic, associated with food-borne human infections and, for epidemiological purposes, can be differentiated by phage typing. The most frequent phage types can be subdivided into genomic groups. Organisms of the prevalent genomic groups and several less frequent ones were mainly associated with poultry transmission and gastroenteritis as the major clinical forms, while organisms of another two frequent groups were mainly associated with extra-intestinal infections, and organisms of four infrequent groups were only collected from sewage or environmental waters.
利用PstI和SphI混合酶进行核糖分型（PS核糖分型）以及使用OPB17引物进行随机扩增多态性DNA（RAPD）分型，对肠炎沙门氏菌进行了分子流行病学研究。对1985年至1996年在西班牙收集的38株食品菌株和25株水源菌株进行了区分，这些菌株在流行病学上无关联，它们被分为20种PS核糖型（鉴别指数[DI]=0.67）、RAPD型（DI=0.28），通过将两种方法结合则分为23个基因组群（DI=0.76）。利用本次研究及之前工作中测试菌株的核糖分型数据，包括临床菌株和参考菌株，绘制了相似性树状图，随后的分支和分组与RAPD型、噬菌体型及来源相关。在相似性水平为55%时，发现了一个主要聚类（包含五个子聚类和三个单分支）和两个次要聚类。结果支持了这样一个事实，即目前至少有40个基因组群的菌株在西班牙传播，但只有五个群的菌株占主导，且它们属于单个子聚类或谱系。这五个群的菌株可被视为地方性菌株，与食源性人类感染有关，出于流行病学目的，可通过噬菌体分型进行区分。最常见的噬菌体型可细分为基因组群。流行基因组群和一些不太常见基因组群的菌株主要与家禽传播及作为主要临床形式的肠胃炎有关，而另外两个常见群的菌株主要与肠道外感染有关，四个不常见群的菌株仅从污水或环境水体中分离得到。
