Deak T, Beuchat L R, Guerzoni M E, Lillie A, Peter G, Rohm H, Schnürer F, Tabajdi P V, Westphal S
Department of Microbiology, University of Horticulture and Food Science, Budapest, Hungary.
Int J Food Microbiol. 1998 Aug 18;43(1-2):91-5. doi: 10.1016/s0168-1605(98)00102-0.
A collaborative study was made to evaluate the effectivity of a general purpose medium, tryptone glucose yeast extract (TGY) agar on the detection and enumeration of yeasts from food. Nine laboratories participated in the study and compared five media (four kinds of TGY with different concentrations of glucose, one of them without tryptone, and, for comparison, dichloran rose bengal chloramphenicol (DRBC) agar). Six food samples were investigated by each laboratory and 23 additional food samples were investigated individually by different laboratories. No difference was found in the performance of media with either the samples common to all laboratories or the various samples tested in different ones. A medium consisting of tryptone, glucose and yeast extract, at any concentration of glucose tested, appeared reliable for the detection and enumeration of yeasts from foods, and its performance did not differ from that of DRBC. Omission of tryptone as recommended by ISO provided an even simpler medium of equally good performance. TGY without chloramphenicol may result in higher total counts due to the development of bacteria. DRBC incubated in light results in lower counts compared to that incubated in the dark.
开展了一项合作研究，以评估通用培养基胰蛋白胨葡萄糖酵母提取物（TGY）琼脂对食品中酵母菌检测和计数的有效性。九个实验室参与了该研究，比较了五种培养基（四种不同葡萄糖浓度的TGY，其中一种不含胰蛋白胨，以及作为对照的孟加拉红氯霉素（DRBC）琼脂）。每个实验室对六个食品样本进行了检测，另外23个食品样本由不同实验室单独检测。对于所有实验室共有的样本以及不同实验室检测的各种样本，各培养基的性能均未发现差异。由胰蛋白胨、葡萄糖和酵母提取物组成的培养基，在所测试的任何葡萄糖浓度下，对食品中酵母菌的检测和计数似乎都是可靠的，其性能与DRBC无异。按照ISO的建议省略胰蛋白胨可提供一种性能同样良好但更简单的培养基。不含氯霉素的TGY可能会因细菌生长而导致总数更高。与在黑暗中培养相比，在光照下培养的DRBC计数更低。
