Nag T C, Jotwani G, Wadhwa S
Department of Anatomy, All India Institute of Medical Sciences, New Delhi.
Neurochem Int. 1998 Aug;33(2):195-200. doi: 10.1016/s0197-0186(98)00004-7.
The localization of taurine in the retina of fetal (12-25 weeks of gestation), postnatal (five-month-old infant) and adult human (35- and 65-year-old) was examined by immunohistochemistry. Additionally, retinas of fresh adult monkey, which served as positive controls, were employed. No immunoreactivity was found in the fetal retinas from 12-15 weeks of gestation. At 1617 weeks of gestation, the ganglion cells and some of their axons were conspicuously labelled for taurine. At 18-19 weeks, Muller glial endfeet, the inner plexiform layer, some amacrine and putative horizontal cells and photoreceptors showed moderate immunoreactivity. With further development at 20-21 and 24-25 weeks of gestation, the immunoreactivity was prominent in Muller cell endfeet, some bipolar cells and in horizontal cells that were aligned in a row in the inner nuclear layer, close to the fovea. At both fetal stages, the photoreceptors and horizontal cells showed strong immunoreactivity. In the postnatal infant retina, taurine immunoreactivity was present in some amacrine cells and photoreceptor inner segments and nuclei, but not in ganglion and horizontal cells, which was also the pattern noted in the adult monkey and human retinas. With development, a shift in the intensity of taurine immunoreactivity was noted towards the outer retina. The expression of taurine immunoreactivity in most fetal retinal neurons implies a role for this amino acid in the normal development as well as maturation of human retina.
采用免疫组织化学方法检测了胎儿（妊娠12 - 25周）、出生后（5个月大婴儿）及成人（35岁和65岁）视网膜中牛磺酸的定位。此外，还使用了新鲜成年猴的视网膜作为阳性对照。在妊娠12 - 15周的胎儿视网膜中未发现免疫反应性。在妊娠16 - 17周时，神经节细胞及其一些轴突被牛磺酸显著标记。在18 - 19周时，米勒神经胶质终足、内网状层、一些无长突细胞、假定的水平细胞和光感受器显示出中度免疫反应性。随着妊娠20 - 21周和24 - 25周的进一步发育，免疫反应性在米勒细胞终足、一些双极细胞以及靠近中央凹的内核层中呈排排列的水平细胞中较为突出。在两个胎儿阶段，光感受器和水平细胞均显示出强免疫反应性。在出生后婴儿的视网膜中，牛磺酸免疫反应性存在于一些无长突细胞以及光感受器的内节和细胞核中，但在神经节细胞和水平细胞中不存在，成年猴和人类视网膜中也呈现这种模式。随着发育，牛磺酸免疫反应性的强度向外层视网膜转移。大多数胎儿视网膜神经元中牛磺酸免疫反应性的表达表明这种氨基酸在人类视网膜的正常发育以及成熟过程中发挥作用。
