来自酿酒酵母的磷酸果糖激酶12S形式的结晶及初步X射线分析。
Crystallization and preliminary X-ray analysis of the 12S form of phosphofructokinase from Saccharomyces cerevisiae.
作者信息
Obmolova G, Kopperschläger G, Heinisch J, Rypniewski W R
机构信息
European Molecular Biology Laboratory (EMBL), c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
出版信息
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):96-8. doi: 10.1107/s0907444997007555.
PMID:9761823
Abstract
The tetrameric 12S form of yeast phosphofructokinase, obtained by limited proteolytic cleavage of the native enzyme, was crystallized under a variety of conditions. The crystals have been characterized in the X-ray beam and are suitable for crystallographic studies.
摘要
通过对天然酵母磷酸果糖激酶进行有限的蛋白水解切割获得的四聚体12S形式，在多种条件下结晶。这些晶体已在X射线束中进行了表征，适合进行晶体学研究。
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