Vassylyev D G, Kashiwagi T, Tomitori H, Kashiwagi K, Igarashi K, Morikawa K
Biomolecular Engineering Research Institute, 6-2-3 Furuedai, Suita, Osaka 565, Japan.
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):132-4. doi: 10.1107/s0907444997009049.
The primary receptor (PotF) of the putrescine transport system in E. coli has been crystallized by the hanging-drop vapor-diffusion technique. The crystals belong to the space group P21212 with unit-cell dimensions a = 269.4, b = 82.33 and c = 93.74 A. The crystals diffract beyond 2.2 A with a rotating-anode X-ray source. A complete data set from the native crystals has been collected and processed at 2.3 A resolution. Two heavy-atom derivatives have been prepared from the same Pt compound at 293 and 277 K. The difference Patterson maps revealed completely different major heavy-atom sites between these two derivatives.
大肠杆菌中腐胺转运系统的主要受体（PotF）已通过悬滴气相扩散技术结晶。晶体属于空间群P21212，晶胞参数为a = 269.4、b = 82.33和c = 93.74 Å。使用旋转阳极X射线源时，晶体的衍射极限为2.2 Å。已收集天然晶体的完整数据集，并在2.3 Å分辨率下进行了处理。在293 K和277 K下，由同一种铂化合物制备了两种重原子衍生物。这两种衍生物的差值帕特森图显示主要重原子位点完全不同。
