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Crystallization of the alpha-hemolysin heptamer solubilized in decyldimethyl- and decyldiethylphosphine oxide.

作者信息

Song L, Gouaux E

机构信息

Department of Biochemistry and Molecular Biology, The University of Chicago, 920 East 58th Street, Chicago, IL 60637, USA.

出版信息

Acta Crystallogr D Biol Crystallogr. 1998 Mar 1;54(Pt 2):276-8. doi: 10.1107/s0907444997010524.

DOI:10.1107/s0907444997010524
PMID:9761893
Abstract

Crystals of the alpha-hemolysin heptamer, a transmembrane pore-forming toxin from Staphylococcus aureus, have been grown using the nonionic detergents n-decyldimethyl- and n-decyldiethylphosphine oxide, phosphorus homologs of the ionic amine oxide detergents. Five crystal forms were obtained, one of which diffracted X-rays to 3 A resolution. This crystal form displayed elements of pseudo mm symmetry in screened precession photographs yet it was triclinic with unit-cell dimensions a = 173, b = 173, c = 102 A, alpha = 92.6, beta = 94.8, gamma = 90.2 degrees.

摘要

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