Shevchik V, Scott M, Mayans O, Jenkins J
Laboratoire de Génétique Moléculaire des Microorganismes, UMR CNRS 5577, INSA Bâtiment 406, 20 avenue Albert Einstein, 69621 Villeurbanne CEDEX, France.
Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):419-22. doi: 10.1107/s0907444997012043.
PeIL, a pectate lyase (E.C. 4.2.2.9) from E. chrysanthemi 3937 that is not homologous to the lyases with known structures, has been purified and crystallized by the hanging-drop method using a variety of organic solvents as precipitant. Elongated lathes grown from poly(ethylene glycol) plus isopropanol belong to the space group P212121 with cell dimensions a = 55.5, b = 58.2, c = 16.4 A with a single molecule in the asymmetric unit. Although complete data sets have been collected to 2.3 A resolution, these crystals diffract to at least 1.9 A resolution and are suitable for structure determination. Chunky plates grown using other organic solvents as the precipitant diffracted to 3 A resolution and were partially characterized as a second orthorhombic crystal form with space group P21212 and cell dimensions a = 119.1, b = 140.5 and c = 105.4 A, suggesting four molecules in the asymmetric unit.
果胶酸裂解酶（PeIL，E.C. 4.2.2.9）来自菊欧文氏菌3937，与已知结构的裂解酶无同源性，已通过悬滴法使用多种有机溶剂作为沉淀剂进行了纯化和结晶。由聚乙二醇加异丙醇生长出的细长板状晶体属于空间群P212121，晶胞参数a = 55.5、b = 58.2、c = 16.4 Å，不对称单元中有一个单分子。尽管已收集到分辨率为2.3 Å的完整数据集，但这些晶体的衍射分辨率至少为1.9 Å，适合进行结构测定。使用其他有机溶剂作为沉淀剂生长出的块状板状晶体衍射分辨率为3 Å，并被部分表征为具有空间群P21212且晶胞参数a = 119.1、b = 140.5和c = 105.4 Å的第二种正交晶型，表明不对称单元中有四个分子。
