Suzuki T, Moriyama H, Hirose R, Sakurai M, Tanaka N, Oshima T
Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 226, Japan.
Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):444-5. doi: 10.1107/s0907444997014133.
3-Isopropylmalate dehydrogenase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7, has been crystallized by the vapor-diffusion method. The crystals were grown from a solution containing ammonium sulfate, 2-methyl-2,4-pentanediol and magnesium chloride. The crystallization requires 2-methyl-2,4-pentanediol to avoid twinning of the crystals. The crystal belongs to the orthorhombic system with the space group P2221 and unit-cell dimensions a = 67.9, b = 93.3 and c = 134.1 A.
来自嗜热嗜酸古菌硫磺矿硫化叶菌属7菌株的3-异丙基苹果酸脱氢酶已通过气相扩散法结晶。晶体从含有硫酸铵、2-甲基-2,4-戊二醇和氯化镁的溶液中生长。结晶需要2-甲基-2,4-戊二醇以避免晶体孪晶。该晶体属于正交晶系，空间群为P2221，晶胞参数a = 67.9、b = 93.3和c = 134.1埃。
