制备性凝胶电泳：从未染色凝胶中检测、切下并洗脱蛋白条带。

Preparative gel electrophoresis: detection, excision, and elution of protein bands from unstained gels.

作者信息

Mardian J K, Isenberg I

机构信息

Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331, USA.

出版信息

Anal Biochem. 1978 Nov;91(1):1-12. doi: 10.1016/0003-2697(78)90811-4.

DOI:10.1016/0003-2697(78)90811-4

Abstract

Methods are described for localizing proteins in unstained gels and accurately excising the regions containing them. A gel elutor, which is all glass with Lucite fittings, is also described. The elutor removes proteins from gels with a high yield and concentrates the protein in a small volume. The elutor is simple and very easy to use. A way is presented for avoiding the oxidation of methionine and cysteine during preparative electrophoresis.

摘要

描述了在未染色凝胶中定位蛋白质并准确切除含有这些蛋白质区域的方法。还描述了一种全玻璃材质、带有有机玻璃配件的凝胶洗脱器。该洗脱器能高效地从凝胶中洗脱蛋白质，并将蛋白质浓缩在小体积中。该洗脱器结构简单，使用非常方便。还介绍了一种在制备性电泳过程中避免甲硫氨酸和半胱氨酸氧化的方法。

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