从蛋白质中去除十二烷基硫酸钠。
Removal of sodium dodecyl sulfate from proteins.
作者信息
Kapp O H, Vinogradov S N
机构信息
Biochemistry Department, Wayne State University School of Medicine, Detroit, Michigan 48201, USA.
出版信息
Anal Biochem. 1978 Nov;91(1):230-5. doi: 10.1016/0003-2697(78)90835-7.
PMID:9762103
Abstract
Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of protein.
摘要
使用离子阻滞树脂AG11A8通过柱色谱法可有效去除水中或磷酸钠缓冲液中蛋白质上的十二烷基硫酸钠。蛋白质的平均回收率为83%，而每摩尔蛋白质上仍残留0.1至1.4摩尔十二烷基硫酸钠。
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