Vrbaski M M, Grujić-Injac B, Gajić D
Institute for Biological Research, Faculty of Science, Belgrade, Yugoslavia.
Anal Biochem. 1978 Nov;91(1):304-8. doi: 10.1016/0003-2697(78)90844-8.
We have established several optimal conditions for qualitative and quantitative allantoin determination by applying Ehrlich's reagent. The limit of detection for allantoin determination amounts to 5 x 10(-6) mM. Allantoin is determined quantitatively by measuring the absorbance at 440 nm (from 300 to 1000 micrograms/ml). The color of the complex becomes stable by standing for 10 min at room temperature. We have used these conditions for allantoin determination in Agrostemma githago seed.
