Monteyne P, Sindic C J
Catholic University of Louvain, Laboratory of Neurochemistry, Brussels, Belgium.
Mult Scler. 1998 Jun;4(3):143-6. doi: 10.1177/135245859800400311.
Reverse transcription polymerase chain reaction (RT-PCR) was used to amplify the mRNA coding for different cytokines in peripheral blood mononuclear cells (PBMC) and cerebrospinal fluid (CSF) cells from 18 multiple sclerosis (MS) patients as compared with 21 other neurological patients. mRNA levels were quantitated by radioactive hybridization of the PCR products. Expression of tumor necrosis factor (TNF)-alpha, interferon (IFN)-gamma, and interleukin (IL)-10 mRNA was elevated in CSF cells of MS patients. In many MS patients, both proinflammatory and immunoregulatory cytokine messages were detected in the CSF compartment. Such immune reactivity of CSF cells, as opposed to PBMC, was not associated with higher clinical activity of the disease. Expression of the B7.1 accessory molecule mRNA was similarly investigated. In the CSF, it was detected only in some clinically active MS cases and in other inflammatory diseases.
与21名其他神经系统疾病患者相比，采用逆转录聚合酶链反应（RT-PCR）扩增了18例多发性硬化症（MS）患者外周血单个核细胞（PBMC）和脑脊液（CSF）细胞中编码不同细胞因子的mRNA。通过PCR产物的放射性杂交对mRNA水平进行定量。MS患者CSF细胞中肿瘤坏死因子（TNF）-α、干扰素（IFN）-γ和白细胞介素（IL）-10 mRNA的表达升高。在许多MS患者中，CSF区室中同时检测到促炎和免疫调节细胞因子信息。与PBMC不同，CSF细胞的这种免疫反应性与疾病的较高临床活性无关。对B7.1辅助分子mRNA的表达进行了类似研究。在CSF中，仅在一些临床活动期MS病例和其他炎症性疾病中检测到。
