Keshgegian A A, Johnston E, Cnaan A
John S. Sharpe Research Foundation and Department of Pathology, The Bryn Mawr Hospital, Pennsylvania 19010, USA.
Am J Clin Pathol. 1998 Oct;110(4):443-9. doi: 10.1093/ajcp/110.4.443.
Predicting the clinical behavior of prostate carcinoma can be difficult; one approach is to identify molecular prognostic markers. We evaluated proliferative rate (MIB-1 antibody) and expression of bcl-2, p53, and retinoblastoma (pRB) proteins, which have cell cycle-related functions, in 208 consecutive radical prostatectomy specimens. Values were correlated with histopathologic parameters (Gleason tumor score, tumor amount, capsule invasion, and involvement of surgical margins, seminal vesicles, or lymph nodes) and with recurrence-free survival (4-year median follow-up). A high MIB-1 proliferative rate was associated with all of the measured histopathologic parameters, p53 overexpression with tumor amount, and pRB expression with positive lymph nodes. pRB and p53 expression levels were not associated with differences in recurrence-free survival. A high MIB-1 proliferative rate and bcl-2 positivity were associated with increased recurrence, both considered individually, and also independently and additively when examined together and with the most predictive histopathologic factors (Gleason tumor score and seminal vesicle involvement). MIB-1 proliferative rate and bcl-2 positivity may prove to be useful markers for poor prognosis in prostate carcinoma.
预测前列腺癌的临床行为可能具有挑战性；一种方法是识别分子预后标志物。我们评估了208例连续前列腺癌根治术标本中的增殖率（MIB-1抗体）以及具有细胞周期相关功能的bcl-2、p53和视网膜母细胞瘤（pRB）蛋白的表达。这些数值与组织病理学参数（Gleason肿瘤评分、肿瘤数量、包膜侵犯以及手术切缘、精囊或淋巴结受累情况）以及无复发生存率（中位随访4年）相关。高MIB-1增殖率与所有测量的组织病理学参数相关，p53过表达与肿瘤数量相关，pRB表达与阳性淋巴结相关。pRB和p53表达水平与无复发生存率的差异无关。高MIB-1增殖率和bcl-2阳性与复发增加相关，单独考虑时如此，一起检查时与最具预测性的组织病理学因素（Gleason肿瘤评分和精囊受累情况）一起时也是独立且相加地具有相关性。MIB-1增殖率和bcl-2阳性可能被证明是前列腺癌预后不良的有用标志物。
