Mor G, Gutierrez L S, Eliza M, Kahyaoglu F, Arici A
Department of Obstetrics and Gynecology, Yale University School of Medicine, New Haven, CT 06520-8063, USA.
Am J Reprod Immunol. 1998 Aug;40(2):89-94. doi: 10.1111/j.1600-0897.1998.tb00396.x.
The low frequency of maternal immune responses to paternally inherited fetal antigens raises the following question: What regulates the immunobiology of pregnancy? Data suggest that this state is the result of peripheral immune-tolerance, an active process of immune-regulation in which activated T cells undergo apoptosis. We studied Fas ligand (FasL) expression and apoptosis in normal and pathologic placentas to find out whether the Fas-FasL-induced apoptosis takes place during implantation.
FasL expression in paraffin sections was detected using specific antibodies and confirmed with reverse transcriptase-polymerase chain reaction of total RNA from frozen placentas. Apoptosis was detected using the terminal deoxy (d)-UTP nick end-labeling assay.
FasL was found in the normal placenta and in gestational trophoblastic disease. Apoptotic leukocytes were localized to the maternal-fetal interface corresponding in localization with the distribution of FasL.
We propose that FasL expression in the placenta is a mechanism responsible for the development of maternal immune tolerance specific for paternal alloantigens and operates in pathologic states characterized by trophoblastic invasion/proliferation.
母体对父系遗传的胎儿抗原产生免疫反应的频率较低，这引发了以下问题：是什么调节了妊娠的免疫生物学？数据表明，这种状态是外周免疫耐受的结果，外周免疫耐受是一种免疫调节的活跃过程，在此过程中活化的T细胞会发生凋亡。我们研究了正常胎盘和病理胎盘组织中Fas配体（FasL）的表达及凋亡情况，以确定Fas - FasL诱导的凋亡是否在植入过程中发生。
使用特异性抗体检测石蜡切片中FasL的表达，并通过对冰冻胎盘总RNA进行逆转录 - 聚合酶链反应加以证实。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法检测凋亡情况。
在正常胎盘和妊娠滋养细胞疾病中均发现了FasL。凋亡白细胞定位于母胎界面，其定位与FasL的分布相对应。
我们认为，胎盘组织中FasL的表达是母体对父系同种异体抗原产生免疫耐受的一种机制，并且在以滋养细胞侵袭/增殖为特征的病理状态中发挥作用。
