Vassileva M, Azcon R, Barea J M, Vassilev N
Estacion Experimental del Zaidin, CSIC, Granada, Spain.
J Biotechnol. 1998 Jul 30;63(1):67-72. doi: 10.1016/s0168-1656(98)00074-1.
Spores of Aspergillus niger were encapsulated in agar, calcium alginate and k-carrageenan and further applied in citric acid production during six repeated batch cultivations. Rock phosphate (RP) at concentrations of 3 g l-1 and 7 g l-1 was supplemented to the culture medium to test encapsulated-fungus solubilizing capability. The highest average citric acid productivity of 0.15 g l-1 h-1 was reached with alginate-bead-encapsulated A. niger on RP-free culture medium while agar seemed to be the most suitable carrier on RP-supplemented medium. Accordingly, the highest average soluble P concentration of 0.20 g l-1 batch-1 was obtained with agar-cell beads as compared with other encapsulated systems.
黑曲霉孢子被包埋在琼脂、海藻酸钙和κ-卡拉胶中，并在六次重复分批培养过程中进一步应用于柠檬酸生产。将浓度为3 g l-1和7 g l-1的磷矿粉（RP）添加到培养基中，以测试包埋真菌的溶解能力。在无RP的培养基上，海藻酸钠珠包埋的黑曲霉平均柠檬酸生产率最高，达到0.15 g l-1 h-1，而在添加RP的培养基上，琼脂似乎是最合适的载体。因此，与其他包埋系统相比，琼脂细胞珠的平均可溶性磷浓度最高，为0.20 g l-1批次-1。
