Kraaij D A, Kamermans M, Spekreijse H
Graduate School Neurosciences Amsterdam, Academic Medical Centre, University of Amsterdam, The Netherlands.
Vis Neurosci. 1998 Sep-Oct;15(5):799-808. doi: 10.1017/s0952523898154184.
The spectral sensitivity of cones in isolated goldfish retina was determined with whole-cell recording techniques. Three spectral classes of cones were found with maximal sensitivities around 620 nm, 540 nm, and 460 nm. UV-cones were not found because our stimulator did not allow effective stimulation in the UV range. The spectral sensitivity of the cones closely matched the cone photopigment absorption spectra at the long wavelength side of the spectrum, but deviated significantly at shorter wavelengths. Surround stimulation induced an inward current in cones due to feedback from horizontal cells. The spectral sensitivity of this feedback signal was determined in all three cone classes and found to be broader than the spectral sensitivity of the cones recorded from, and to be spectrally nonopponent. These data are consistent with a connectivity scheme between cones and horizontal cells in which the three horizontal cell systems feed back to all cone systems and in which all horizontal cell systems receive input from more than one cone system.
采用全细胞记录技术测定了分离的金鱼视网膜中视锥细胞的光谱敏感性。发现了三类视锥细胞，其最大敏感性分别在620纳米、540纳米和460纳米左右。未发现紫外视锥细胞，因为我们的刺激器不允许在紫外波段进行有效刺激。视锥细胞的光谱敏感性在光谱的长波长一侧与视锥细胞光色素吸收光谱紧密匹配，但在较短波长处有显著偏差。周围刺激由于水平细胞的反馈而在视锥细胞中诱发内向电流。在所有三类视锥细胞中都测定了这种反馈信号的光谱敏感性，发现其比所记录的视锥细胞的光谱敏感性更宽，且在光谱上是非对立的。这些数据与视锥细胞和水平细胞之间的连接模式一致，即三个水平细胞系统反馈到所有视锥细胞系统，且所有水平细胞系统都从不止一个视锥细胞系统接收输入。
