Salazar I, Sánchez Quinteiro P
Departamento de Anatomía y Embriología, Facultad de Veterinaria de Lugo, Universidad de Santiago de Compostela, Spain.
Anat Embryol (Berl). 1998 Oct;198(4):331-9. doi: 10.1007/s004290050188.
A number of previous studies have indicated that lectin histochemistry is an obvious choice for characterizing the vomeronasal system. However, apparently inconsistent results have been obtained: notably, the affinity with which various lectins bind to the accessory olfactory bulb varies among taxa, even considering closely related species. In the present study, the binding patterns of seven lectins in the rat accessory olfactory bulb, vomeronasal nerves and vomeronasal duct were investigated. The Bandeiraea simplicifolia lectin bound exclusively to the vomeronasal nerve and glomerular layers of the accessory olfactory bulb, while the Ulex europeus and Lycopersicon esculentum lectins bound to these regions and additionally to the nerve and glomerular layers of the main olfactory bulb. Soybean agglutinin showed a similar pattern to that obtained with the Ulex europeus and Lycopersicon esculentum lectins, though it also faintly labelled other parts of the structures examined. The Vicia villosa and Erythrina cristagalli lectins were not specific for the vomeronasal system, since they labelled grey and white matters in structures including the lateral olfactory tract and the anterior olfactory nuclei. The Dolichos biflorus lectin did not bind to vomeronasal tissues. The observed patterns of binding in the accessory olfactory bulb were consistent with those observed in the vomeronasal nerves, but unlike those observed in the epithelium of the vomeronasal duct. This latter result probably reflects binding of lectins to sugar residues contained in secreted mucus rather than those in epithelial nerve endings.
此前的多项研究表明，凝集素组织化学是表征犁鼻系统的一个明显选择。然而，已获得的结果显然不一致：值得注意的是，即使考虑亲缘关系很近的物种，各种凝集素与副嗅球结合的亲和力在不同分类群中也有所不同。在本研究中，研究了七种凝集素在大鼠副嗅球、犁鼻神经和犁鼻管中的结合模式。简单叶刀豆凝集素仅与犁鼻神经和副嗅球的肾小球层结合，而欧洲荆豆凝集素和番茄凝集素则与这些区域结合，此外还与主嗅球的神经和肾小球层结合。大豆凝集素显示出与欧洲荆豆凝集素和番茄凝集素相似的模式，不过它也微弱地标记了所检查结构的其他部分。绒毛野豌豆凝集素和刺桐凝集素对犁鼻系统不具有特异性，因为它们标记了包括外侧嗅束和前嗅核在内的结构中的灰质和白质。双花扁豆凝集素不与犁鼻组织结合。在副嗅球中观察到的结合模式与在犁鼻神经中观察到的一致，但与在犁鼻管上皮中观察到的不同。后一结果可能反映了凝集素与分泌黏液中所含糖残基的结合，而非与上皮神经末梢中糖残基的结合。
