Moutafchiev D A, Sirakov L M
Department of Biochemistry, Medical Faculty, Medical University, Sofia, Bulgaria.
Biol Trace Elem Res. 1998 Jul;63(1):31-6. doi: 10.1007/BF02785275.
The effects of ascorbic acid, sodium citrate, and sodium bicarbonate on 59Fe-transferrin, 54Mn-transferrin, and 65Zn-transferrin uptake by the receptors disposed of plasma membrane isolated from lactating mouse mammary gland cells have been investigated. The effect of 10(-2) mol/L ascorbic acid alone and in combination with NaHCO3 on the 59Fe-transferrin uptake is significant and positive. 54Mn-transferrin and 65Zn-transferrin binding to the cell receptors are influenced optimally by 0.5 mol/L sodium bicarbonate. Sodium citrate alone or in combination with other substances always has a negative effect on binding of these three metals. It is suggested that a precise mechanism may exist with large possibilities to rearrange metal uptake and its transport from blood to milk.
研究了抗坏血酸、柠檬酸钠和碳酸氢钠对从泌乳小鼠乳腺细胞分离的质膜上的受体摄取59Fe-转铁蛋白、54Mn-转铁蛋白和65Zn-转铁蛋白的影响。单独的10(-2)mol/L抗坏血酸以及与NaHCO3联合使用对59Fe-转铁蛋白摄取的影响显著且呈正向作用。0.5mol/L碳酸氢钠对54Mn-转铁蛋白和65Zn-转铁蛋白与细胞受体的结合影响最佳。单独的柠檬酸钠或与其他物质联合使用时，对这三种金属的结合总是产生负面影响。提示可能存在一种精确的机制，极有可能重新调整金属的摄取及其从血液到乳汁的转运。
