Kolassa N, Turnheim K
Experientia. 1976 Oct 15;32(10):1355-6. doi: 10.1007/BF01953138.
The applicability of the direct linear plot is compared with that of the Scatchard plot for the estimation of protein binding parameters. Only, if one class of binding sites exists in the system tested, binding parameters may be estimated by use of the direct linear plot. On the other hand the Scatchard plot also provides estimates in systems with more that one class of binding sites.
比较直接线性图与Scatchard图在估算蛋白质结合参数方面的适用性。只有当所测试的系统中存在一类结合位点时,才可以使用直接线性图来估算结合参数。另一方面,Scatchard图也能为具有不止一类结合位点的系统提供估算值。