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直接线性图在评估药物-蛋白质结合参数方面的局限性。

Limitations of the direct linear plot in evaluation of drug-protein binding parameters.

作者信息

Kolassa N, Turnheim K

出版信息

Experientia. 1976 Oct 15;32(10):1355-6. doi: 10.1007/BF01953138.

DOI:10.1007/BF01953138
PMID:976464
Abstract

The applicability of the direct linear plot is compared with that of the Scatchard plot for the estimation of protein binding parameters. Only, if one class of binding sites exists in the system tested, binding parameters may be estimated by use of the direct linear plot. On the other hand the Scatchard plot also provides estimates in systems with more that one class of binding sites.

摘要

比较直接线性图与Scatchard图在估算蛋白质结合参数方面的适用性。只有当所测试的系统中存在一类结合位点时,才可以使用直接线性图来估算结合参数。另一方面,Scatchard图也能为具有不止一类结合位点的系统提供估算值。

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本文引用的文献

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DETERMINATION OF EQUILIBRIUM CONSTANTS AND MAXIMUM BINDING CAPACITIES IN COMPLEX IN VITRO SYSTEMS. I. THE MAMMILLARY SYSTEM.
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Properties of graphical representations of multiple classes of binding sites.多类结合位点的图形表示特性。
Biochemistry. 1971 Aug 3;10(16):3065-9. doi: 10.1021/bi00792a013.
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The binding of drugs by plasma proteins.药物与血浆蛋白的结合。
J Pharm Sci. 1968 Jun;57(6):895-918. doi: 10.1002/jps.2600570601.
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The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.直接线性作图法。一种用于估算酶动力学参数的新的图形方法。
Biochem J. 1974 Jun;139(3):715-20. doi: 10.1042/bj1390715.
6
Statistical considerations in the estimation of enzyme kinetic parameters by the direct linear plot andother methods.通过直接线性作图法及其他方法估算酶动力学参数时的统计学考量
Biochem J. 1974 Jun;139(3):721-30. doi: 10.1042/bj1390721.
7
An unbiased method for estimating binding parameters in a non-cooperative binding process.
Biochim Biophys Acta. 1974 Dec 18;371(2):482-90. doi: 10.1016/0005-2795(74)90044-0.