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利用视频成像评估植物次生化合物对曼氏血吸虫毛蚴的亚致死影响。

The use of video-imaging to assess the sub-lethal impact of plant secondary compounds on Schistosoma mansoni miracidia.

作者信息

Lyddiard J R, Bartlett A, Gray B, Whitfield P J

机构信息

Infection and Immunity Research Group, Division of Life Sciences, King's College London, University of London, Campden Hill Road, Kensington, London, W8 7AH, UK.

出版信息

J Helminthol. 1998 Sep;72(3):237-41. doi: 10.1017/s0022149x00016503.

DOI:10.1017/s0022149x00016503
PMID:9765376
Abstract

The study describes methods developed for using video-imaging technology to record and measure the velocity of Schistosoma mansoni miracidia. The efficacy of the classical bioassay procedure (a qualitative behavioural assay) was compared with that of the new quantitative protocol, for assessing the sub-lethal impact of a larvicidal dichloromethane extract of the seeds of Millettia thonningii on miracidia. The new technique confirmed the efficacy of the classical bioassay for rapid determination of the lethal and sub-lethal impact of larvicides but also provided quantitative information on sub-lethal impacts on miracidial velocity and shape.

摘要

该研究描述了为使用视频成像技术记录和测量曼氏血吸虫毛蚴速度而开发的方法。将经典生物测定程序(一种定性行为测定)的功效与新的定量方案进行了比较,以评估厚果鸡血藤种子的杀幼虫二氯甲烷提取物对毛蚴的亚致死影响。新技术证实了经典生物测定法在快速确定杀幼虫剂的致死和亚致死影响方面的功效,同时还提供了关于对毛蚴速度和形状的亚致死影响的定量信息。

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