爱泼斯坦-巴尔病毒小RNA（EBER）基因：在病毒裂解复制过程中的差异调控
Epstein-Barr virus small RNA (EBER) genes: differential regulation during lytic viral replication.
作者信息
Greifenegger N, Jäger M, Kunz-Schughart L A, Wolf H, Schwarzmann F
机构信息
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg, Germany.
出版信息
J Virol. 1998 Nov;72(11):9323-8. doi: 10.1128/JVI.72.11.9323-9328.1998.
Abstract
In every latently Epstein-Barr virus-infected cell the viral genes EBER-1 and EBER-2 are transcribed by polymerase III. In lytically infected cells in vivo the EBER genes could not be detected. However, in cell culture downregulation could not be confirmed, and hence the relevance of this shutdown to the replication of the virus was not clear. We assayed the transcriptional activity of the EBER genes by nuclear run-on assays with enriched lytically infected cells and demonstrated that EBER-1 and EBER-2 are differentially downregulated on the transcriptional level during the switch to lytic viral replication. This downregulation was an early event during the lytic replication of the virus.
摘要
在每一个潜伏感染爱泼斯坦-巴尔病毒的细胞中，病毒基因EBER-1和EBER-2由聚合酶III转录。在体内处于裂解感染状态的细胞中未检测到EBER基因。然而，在细胞培养中，这种下调未能得到证实，因此这种关闭对病毒复制的相关性尚不清楚。我们通过对富集的裂解感染细胞进行核延伸分析来检测EBER基因的转录活性，并证明在向裂解性病毒复制转变过程中，EBER-1和EBER-2在转录水平上存在差异下调。这种下调是病毒裂解复制过程中的早期事件。
相似文献
1
Epstein-Barr virus small RNA (EBER) genes: differential regulation during lytic viral replication.爱泼斯坦-巴尔病毒小RNA（EBER）基因：在病毒裂解复制过程中的差异调控
J Virol. 1998 Nov;72(11):9323-8. doi: 10.1128/JVI.72.11.9323-9328.1998.
2
CpG-methylation silences the activity of the RNA polymerase III transcribed EBER-1 promoter of Epstein-Barr virus.CpG甲基化使爱泼斯坦-巴尔病毒的RNA聚合酶III转录的EBER-1启动子的活性沉默。
FEBS Lett. 2008 Mar 5;582(5):705-9. doi: 10.1016/j.febslet.2008.01.042. Epub 2008 Feb 5.
3
Variant chromatin structure of the oriP region of Epstein-Barr virus and regulation of EBER1 expression by upstream sequences and oriP.爱泼斯坦-巴尔病毒oriP区域的可变染色质结构以及上游序列和oriP对EBER1表达的调控
J Virol. 2001 Jul;75(13):6235-41. doi: 10.1128/JVI.75.13.6235-6241.2001.
4
The SWI/SNF Chromatin Regulator BRG1 Modulates the Transcriptional Regulatory Activity of the Epstein-Barr Virus DNA Polymerase Processivity Factor BMRF1.SWI/SNF染色质调节因子BRG1调节爱泼斯坦-巴尔病毒DNA聚合酶持续合成因子BMRF1的转录调节活性。
J Virol. 2017 Apr 13;91(9). doi: 10.1128/JVI.02114-16. Print 2017 May 1.
5
Recombinant Epstein-Barr virus with small RNA (EBER) genes deleted transforms lymphocytes and replicates in vitro.缺失小RNA（EBER）基因的重组爱泼斯坦-巴尔病毒可转化淋巴细胞并在体外复制。
Proc Natl Acad Sci U S A. 1991 Feb 15;88(4):1546-50. doi: 10.1073/pnas.88.4.1546.
6
Host Gene Expression Is Regulated by Two Types of Noncoding RNAs Transcribed from the Epstein-Barr Virus BamHI A Rightward Transcript Region.宿主基因表达受源自爱泼斯坦-巴尔病毒BamHI A向右转录区的两种非编码RNA调控。
J Virol. 2015 Nov;89(22):11256-68. doi: 10.1128/JVI.01492-15. Epub 2015 Aug 26.
7
Expression of genes for the Epstein-Barr virus small RNAs EBER-1 and EBER-2 in Daudi Burkitt's lymphoma cells: effects of interferon treatment.爱泼斯坦-巴尔病毒小RNA EBER-1和EBER-2基因在Daudi伯基特淋巴瘤细胞中的表达：干扰素治疗的影响
J Gen Virol. 1992 Dec;73 ( Pt 12):3169-75. doi: 10.1099/0022-1317-73-12-3169.
8
Epstein-Barr virus gene expression in interferon-treated cells. Implications for the regulation of protein synthesis and the antiviral state.干扰素处理细胞中的爱泼斯坦-巴尔病毒基因表达。对蛋白质合成调控和抗病毒状态的影响。
Biochim Biophys Acta. 1990 Aug 27;1050(1-3):167-73. doi: 10.1016/0167-4781(90)90161-t.
9
Epstein-Barr virus replicative gene transcription during de novo infection of human thymocytes: simultaneous early expression of BZLF-1 and its repressor RAZ.人胸腺细胞初次感染期间的爱泼斯坦-巴尔病毒复制基因转录：BZLF-1及其抑制因子RAZ的同时早期表达
Virology. 1995 Apr 20;208(2):685-95. doi: 10.1006/viro.1995.1200.
10
Relative levels of EBNA1 gene transcripts from the C/W, F and Q promoters in Epstein-Barr virus-transformed lymphoid cells in latent and lytic stages of infection.在爱泼斯坦-巴尔病毒感染的潜伏和裂解阶段，来自C/W、F和Q启动子的EBNA1基因转录本在爱泼斯坦-巴尔病毒转化的淋巴样细胞中的相对水平。
J Gen Virol. 1999 Feb;80 ( Pt 2):457-466. doi: 10.1099/0022-1317-80-2-457.
引用本文的文献
1
The Prevalence of Epstein-Barr Virus in Plasma Cell Neoplasms is Higher in HIV-Positive Individuals.在 HIV 阳性个体中，浆细胞肿瘤中 Epstein-Barr 病毒的流行率更高。
Int J Surg Pathol. 2023 Aug;31(5):564-571. doi: 10.1177/10668969221113490. Epub 2022 Aug 1.
2
Virus-Mediated Inhibition of Apoptosis in the Context of EBV-Associated Diseases: Molecular Mechanisms and Therapeutic Perspectives.病毒介导的凋亡抑制在 EBV 相关疾病中的作用：分子机制和治疗展望。
Int J Mol Sci. 2022 Jun 30;23(13):7265. doi: 10.3390/ijms23137265.
3
Inhibition of PKR by Viruses.病毒对蛋白激酶R的抑制作用。
Front Microbiol. 2021 Oct 25;12:757238. doi: 10.3389/fmicb.2021.757238. eCollection 2021.
4
Uncovering early events in primary Epstein-Barr virus infection using a rabbit model.利用兔模型揭示原发性 EBV 感染的早期事件。
Sci Rep. 2021 Oct 27;11(1):21220. doi: 10.1038/s41598-021-00668-x.
5
Distinct pattern of lymphoid neoplasms characterizations according to the WHO classification (2016) and prevalence of associated Epstein-Barr virus infection in Nigeria population.根据世界卫生组织（2016年）分类法对淋巴瘤的不同特征模式以及尼日利亚人群中相关爱泼斯坦-巴尔病毒感染的患病率。
Infect Agent Cancer. 2021 May 24;16(1):36. doi: 10.1186/s13027-021-00378-z.
6
Epstein-Barr Virus Reactivation-Induced Immunoglobulin Production: Significance on Autoimmunity.爱泼斯坦-巴尔病毒再激活诱导的免疫球蛋白产生：对自身免疫的意义
Microorganisms. 2020 Nov 27;8(12):1875. doi: 10.3390/microorganisms8121875.
7
Rapid identification and characterization of infected cells in blood during chronic active Epstein-Barr virus infection.在慢性活动性 EBV 感染期间快速鉴定和特征化血液中的感染细胞。
J Exp Med. 2020 Nov 2;217(11). doi: 10.1084/jem.20192262.
8
Viral non-coding RNAs: Stealth strategies in the tug-of-war between humans and herpesviruses.病毒非编码 RNA：人类与疱疹病毒之间拉锯战中的隐匿策略。
Semin Cell Dev Biol. 2021 Mar;111:135-147. doi: 10.1016/j.semcdb.2020.06.015. Epub 2020 Jul 3.
9
Mapping of human papilloma virus, p16, and epstein-barr virus in non-malignant tonsillar disease.人乳头瘤病毒、p16和爱泼斯坦-巴尔病毒在非恶性扁桃体疾病中的定位
Laryngoscope Investig Otolaryngol. 2019 Apr 12;4(3):285-291. doi: 10.1002/lio2.260. eCollection 2019 Jun.
10
Opportunities to Target the Life Cycle of Epstein-Barr Virus (EBV) in EBV-Associated Lymphoproliferative Disorders.靶向爱泼斯坦-巴尔病毒（EBV）相关淋巴增殖性疾病中EBV生命周期的机会。
Front Oncol. 2019 Mar 15;9:127. doi: 10.3389/fonc.2019.00127. eCollection 2019.
本文引用的文献
1
The control of lytic replication of Epstein-Barr virus in B lymphocytes (Review).爱泼斯坦-巴尔病毒在B淋巴细胞中裂解性复制的控制（综述）
Int J Mol Med. 1998 Jan;1(1):137-42. doi: 10.3892/ijmm.1.1.137.
2
Striking similarities are exhibited by two small Epstein-Barr virus-encoded ribonucleic acids and the adenovirus-associated ribonucleic acids VAI and VAII.两种小的爱泼斯坦-巴尔病毒编码的核糖核酸与腺病毒相关核糖核酸VAI和VAII表现出惊人的相似性。
Mol Cell Biol. 1981 Sep;1(9):785-96. doi: 10.1128/mcb.1.9.785-796.1981.
3
4
Epstein-Barr virus (EBV) infection in salivary gland tumors: lytic EBV infection in nonmalignant epithelial cells surrounded by EBV-positive T-lymphoma cells.唾液腺肿瘤中的爱泼斯坦-巴尔病毒（EBV）感染：EBV阳性T淋巴瘤细胞包围的非恶性上皮细胞中的EBV裂解性感染。
Virology. 1997 Jan 20;227(2):484-7. doi: 10.1006/viro.1996.8352.
5
Detection of Epstein-Barr virus transcripts in chemically or immunologically-activated cells and in a null cell-line (HLN-STL-C) by in situ hybridization with alkaline phosphatase-linked oligonucleotide probes.通过与碱性磷酸酶连接的寡核苷酸探针进行原位杂交，检测化学或免疫激活细胞以及空细胞系（HLN-STL-C）中的爱泼斯坦-巴尔病毒转录本。
J Virol Methods. 1993 Oct;44(2-3):141-54. doi: 10.1016/0166-0934(93)90050-2.
6
Quantitative polymerase chain reaction analysis of mdr1 mRNA in multiple myeloma cell lines and clinical specimens.多发性骨髓瘤细胞系及临床标本中mdr1 mRNA的定量聚合酶链反应分析
Anal Biochem. 1993 Sep;213(2):414-21. doi: 10.1006/abio.1993.1440.
7
The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes.爱泼斯坦-巴尔病毒（EBV）小RNA EBER1在EBV感染的人类B淋巴细胞中与核糖体蛋白L22结合并使其重新定位。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3463-7. doi: 10.1073/pnas.91.8.3463.
8
Negatively cis-acting elements in the distal part of the promoter of Epstein-Barr virus trans-activator gene BZLF1.爱泼斯坦-巴尔病毒反式激活基因BZLF1启动子远端的负向顺式作用元件。
J Gen Virol. 1994 Aug;75 ( Pt 8):1999-2006. doi: 10.1099/0022-1317-75-8-1999.
9
Epstein-Barr virus latency in blood mononuclear cells: analysis of viral gene transcription during primary infection and in the carrier state.爱泼斯坦-巴尔病毒在血液单核细胞中的潜伏：原发性感染及携带状态下病毒基因转录分析
J Virol. 1994 Nov;68(11):7374-85. doi: 10.1128/JVI.68.11.7374-7385.1994.
10
Comparative analysis of the regulation of the interferon-inducible protein kinase PKR by Epstein-Barr virus RNAs EBER-1 and EBER-2 and adenovirus VAI RNA.爱泼斯坦-巴尔病毒RNA EBER-1和EBER-2以及腺病毒VAI RNA对干扰素诱导蛋白激酶PKR调控的比较分析
Nucleic Acids Res. 1993 Sep 25;21(19):4483-90. doi: 10.1093/nar/21.19.4483.
Zotero 插件