Greifenegger N, Jäger M, Kunz-Schughart L A, Wolf H, Schwarzmann F
Institut für Medizinische Mikrobiologie und Hygiene, Universität Regensburg, Regensburg, Germany.
J Virol. 1998 Nov;72(11):9323-8. doi: 10.1128/JVI.72.11.9323-9328.1998.
In every latently Epstein-Barr virus-infected cell the viral genes EBER-1 and EBER-2 are transcribed by polymerase III. In lytically infected cells in vivo the EBER genes could not be detected. However, in cell culture downregulation could not be confirmed, and hence the relevance of this shutdown to the replication of the virus was not clear. We assayed the transcriptional activity of the EBER genes by nuclear run-on assays with enriched lytically infected cells and demonstrated that EBER-1 and EBER-2 are differentially downregulated on the transcriptional level during the switch to lytic viral replication. This downregulation was an early event during the lytic replication of the virus.
在每一个潜伏感染爱泼斯坦-巴尔病毒的细胞中，病毒基因EBER-1和EBER-2由聚合酶III转录。在体内处于裂解感染状态的细胞中未检测到EBER基因。然而，在细胞培养中，这种下调未能得到证实，因此这种关闭对病毒复制的相关性尚不清楚。我们通过对富集的裂解感染细胞进行核延伸分析来检测EBER基因的转录活性，并证明在向裂解性病毒复制转变过程中，EBER-1和EBER-2在转录水平上存在差异下调。这种下调是病毒裂解复制过程中的早期事件。
