Bath C, Dyall-Smith M L
Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052, Australia.
J Virol. 1998 Nov;72(11):9392-5. doi: 10.1128/JVI.72.11.9392-9395.1998.
A novel archaeal virus, His1, was isolated from hypersaline waters in southeastern Australia. It was lytic, grew only on Haloarcula hispanica (titers of up to 10(11) PFU/ml), and displayed a lemon-shaped morphology (74 by 44 nm) previously reported only for a virus of the extreme thermophiles (SSV1). The density of His1 was approximately 1.28 g/ml, similar to that of SSV1 (1.24 g/ml). Purified particles were resistant to low salt concentrations. The genome was linear, double-stranded DNA of 14.9 kb, similar to the genome of SSV1 (15.5 kb). Morphologically, this isolate clearly belongs to the recently proposed Fuselloviridae family of archaeal viruses. It is the first member of this family from the extremely halophilic archaea, and its host, H. hispanica, can be readily manipulated genetically.
一种新型古病毒His1,是从澳大利亚东南部的高盐水域中分离出来的。它具有裂解性,仅在西班牙盐盒菌(Haloarcula hispanica)上生长(滴度高达10¹¹ PFU/ml),呈现出柠檬形形态(74×44纳米),此前仅在嗜热古菌病毒(SSV1)中报道过这种形态。His1的密度约为1.28 g/ml,与SSV1的密度(1.24 g/ml)相似。纯化后的病毒颗粒对低盐浓度具有抗性。其基因组为14.9 kb的线性双链DNA,与SSV1的基因组(15.5 kb)相似。从形态学上看,这种分离物显然属于最近提出的古病毒梭形病毒科(Fuselloviridae)。它是该科来自极端嗜盐古菌的首个成员,其宿主西班牙盐盒菌在遗传学上易于操作。