Tamura M, Kuwano K, Kurane I, Ennis F A
Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical Center, Worcester, Massachusetts, USA.
J Virol. 1998 Nov;72(11):9404-6. doi: 10.1128/JVI.72.11.9404-9406.1998.
We defined the epitopes recognized by three influenza A virus-specific, H-2Kd-restricted CD8(+) cytotoxic T-lymphocyte (CTL) clones: H1-specific clone A-12, H2-specific clone F-4, and H1- and H2-cross-reactive clone B7-B7. The A-12 and B7-B7 clones recognized the same peptide, which comprises amino acids 533 to 541 (IYSTVASSL) of A/PR/8 hemagglutinin (HA). The F-4 and B7-B7 clones both recognized the peptide which comprise amino acids 529 to 537 (IYATVAGSL) of A/Jap HA. Amino acids 533 to 541 of A/PR/8 HA are compatible with amino acids 529 to 537 of A/Jap HA. Amino acid S at positions 3 and 7 was responsible for recognition by H1-specific clone A-12, while amino acid G at position 7 was responsible for recognition by H2-specific clone F-4. Two conserved amino acids, T at position 4 and A at position 6, were responsible for recognition by H1-, and H2-cross-reactive clone B7-B7. These results indicate that a single nine-amino-acid region is recognized by HA-specific CTL clones of three different subtype specificities and that the amino acids responsible for the recognition by the CTL clones are different.
我们定义了三种甲型流感病毒特异性、H-2Kd限制性CD8(+)细胞毒性T淋巴细胞(CTL)克隆所识别的表位：H1特异性克隆A-12、H2特异性克隆F-4以及H1和H2交叉反应性克隆B7-B7。A-12和B7-B7克隆识别相同的肽段，该肽段由A/PR/8血凝素(HA)的第533至541位氨基酸(IYSTVASSL)组成。F-4和B7-B7克隆均识别由A/Jap HA的第529至537位氨基酸(IYATVAGSL)组成的肽段。A/PR/8 HA的第533至541位氨基酸与A/Jap HA的第529至537位氨基酸相符。第3位和第7位的氨基酸S负责被H1特异性克隆A-12识别，而第7位的氨基酸G负责被H2特异性克隆F-4识别。两个保守氨基酸，第4位的T和第6位的A，负责被H1和H2交叉反应性克隆B7-B7识别。这些结果表明，一个单一的九氨基酸区域被三种不同亚型特异性的HA特异性CTL克隆所识别，并且负责被CTL克隆识别的氨基酸是不同的。
