IS1411的鉴定与特性分析，IS1411是一种新的插入序列，可导致恶臭假单胞菌中苯酚降解基因的转录激活。
Identification and characterization of IS1411, a new insertion sequence which causes transcriptional activation of the phenol degradation genes in Pseudomonas putida.
作者信息
Kallastu A, Hõrak R, Kivisaar M
机构信息
Estonian Biocentre and Institute of Molecular and Cell Biology, Tartu University, EE2400 Tartu, Estonia.
出版信息
J Bacteriol. 1998 Oct;180(20):5306-12. doi: 10.1128/JB.180.20.5306-5312.1998.
Abstract
A new insertion sequence (IS element), IS1411, was identified downstream of the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas sp. strain EST1001. According to sequence analysis, IS1411 belongs to a new family of IS elements that has recently been named the ISL3 family (J. Mahillon and M. Chandler, Microbiol. Mol. Biol. Rev. 62:725-774, 1998). IS1411 generates 8-bp duplication of the target DNA and carries 24-bp inverted repeats (IRs), highly homologous to the IRs of other IS elements belonging to this family. IS1411 was discovered as a result of insertional activation of promoterless pheBA genes in Pseudomonas putida due to the presence of outward-directed promoters at the left end of IS1411. Both promoters located on the IS element have sequences that are similar to the consensus sequence of Escherichia coli sigma70. IS1411 can produce IS circles, and the circle formation is enhanced when two copies of the element are present in the same plasmid.
摘要
一个新的插入序列（IS元件）IS1411，在源自假单胞菌属菌株EST1001质粒DNA的苯酚降解基因pheBA下游被鉴定出来。根据序列分析，IS1411属于一个最近被命名为ISL3家族的新的IS元件家族（J. 马希隆和M. 钱德勒，《微生物学与分子生物学综述》62:725 - 774，1998年）。IS1411会使靶DNA产生8个碱基对的重复，并带有24个碱基对的反向重复序列（IRs），与属于该家族的其他IS元件的IRs高度同源。IS1411是由于在恶臭假单胞菌中无启动子的pheBA基因的插入激活而被发现的，这是因为IS1411左端存在向外的启动子。位于该IS元件上的两个启动子的序列都与大肠杆菌σ70的共有序列相似。IS1411能够产生IS环，并且当元件的两个拷贝存在于同一质粒中时，环的形成会增强。
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