Herrmann H, Müller C, Schmidt I, Mahnke J, Petruschka L, Hahnke K
Ernst-Moritz-Arndt-Universität Greifswald, Institut für Genetik und Biochemie der Fachrichtung Biologie, Germany.
Mol Gen Genet. 1995 Apr 20;247(2):240-6. doi: 10.1007/BF00705655.
The genetic organization of the DNA region encoding the phenol degradation pathway of Pseudomonas putida H has been investigated. This strain can utilize phenol or some of its methylated derivatives as its sole source of carbon and energy. The first step in this process is the conversion of phenol into catechol. Catechol is then further metabolized via the meta-cleavage pathway into TCA cycle intermediates. Genes encoding these enzymes are clustered on the plasmid pPGH1. A region of contiguous DNA spanning about 16 kb contains all of the genetic information necessary for inducible phenol degradation. The analysis of mutants generated by insertion of transposons and cassettes indicates that all of the catabolic genes are contained in a single operon. This codes for a multicomponent phenol hydroxylase and meta-cleavage pathway enzymes. Catabolic genes are subject to positive control by the gene product(s) of a second locus.
已对恶臭假单胞菌H编码苯酚降解途径的DNA区域的遗传组织进行了研究。该菌株能够利用苯酚或其某些甲基化衍生物作为唯一的碳源和能源。此过程的第一步是将苯酚转化为儿茶酚。然后，儿茶酚通过间位裂解途径进一步代谢为三羧酸循环中间体。编码这些酶的基因聚集在质粒pPGH1上。一段约16 kb的连续DNA区域包含诱导型苯酚降解所需的所有遗传信息。对通过转座子和盒式插入产生的突变体的分析表明，所有分解代谢基因都包含在一个操纵子中。这编码一种多组分苯酚羟化酶和间位裂解途径酶。分解代谢基因受到第二个基因座的基因产物的正调控。
