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pTF-FC2蛋白质毒素-抗毒素质粒成瘾系统（pas）的自动调节对于质粒稳定至关重要。

Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization.

作者信息

Smith A S, Rawlings D E

机构信息

Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.

出版信息

J Bacteriol. 1998 Oct;180(20):5463-5. doi: 10.1128/JB.180.20.5463-5465.1998.

DOI:10.1128/JB.180.20.5463-5465.1998
PMID:9765582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107599/
Abstract

The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.

摘要

广宿主范围质粒pTF-FC2的蛋白质质粒成瘾系统的pasABC基因受到自身调控。解毒蛋白PasA能够单独将操纵子抑制25倍，当也存在毒素蛋白PasB时，抑制作用增加到100倍。自身调控似乎是pas介导的质粒稳定所必需的，因为当pas基因置于异丙基-β-D-硫代半乳糖苷（IPTG）调控的tac启动子之后时，它们无法稳定异源测试质粒。

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本文引用的文献

1
Efficiency of the pTF-FC2 pas poison-antidote stability system in Escherichia coli is affected by the host strain, and antidote degradation requires the lon protease.pTF-FC2 pas 毒素-抗毒素稳定性系统在大肠杆菌中的效率受宿主菌株影响，且抗毒素的降解需要lon蛋白酶。
J Bacteriol. 1998 Oct;180(20):5458-62. doi: 10.1128/JB.180.20.5458-5462.1998.
2
The poison-antidote stability system of the broad-host-range Thiobacillus ferrooxidans plasmid pTF-FC2.广宿主范围的氧化亚铁硫杆菌质粒pTF-FC2的毒剂-解毒剂稳定系统
Mol Microbiol. 1997 Dec;26(5):961-70. doi: 10.1046/j.1365-2958.1997.6332000.x.
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Characteristics and significance of DNA binding activity of plasmid stabilization protein ParD from the broad host-range plasmid RK2.来自广宿主范围质粒RK2的质粒稳定蛋白ParD的DNA结合活性的特征及意义。
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Molecular genetics of Thiobacillus ferrooxidans.氧化亚铁硫杆菌的分子遗传学
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