pTF-FC2蛋白质毒素-抗毒素质粒成瘾系统（pas）的自动调节对于质粒稳定至关重要。
Autoregulation of the pTF-FC2 proteic poison-antidote plasmid addiction system (pas) is essential for plasmid stabilization.
作者信息
Smith A S, Rawlings D E
机构信息
Department of Microbiology, University of Cape Town, Rondebosch 7700, South Africa.
出版信息
J Bacteriol. 1998 Oct;180(20):5463-5. doi: 10.1128/JB.180.20.5463-5465.1998.
Abstract
The pasABC genes of the proteic plasmid addiction system of broad-host-range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appears to be an essential requirement for pas-mediated plasmid stabilization because when the pas genes were placed behind the isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.
摘要
广宿主范围质粒pTF-FC2的蛋白质质粒成瘾系统的pasABC基因受到自身调控。解毒蛋白PasA能够单独将操纵子抑制25倍，当也存在毒素蛋白PasB时，抑制作用增加到100倍。自身调控似乎是pas介导的质粒稳定所必需的，因为当pas基因置于异丙基-β-D-硫代半乳糖苷（IPTG）调控的tac启动子之后时，它们无法稳定异源测试质粒。
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