从黑腹果蝇中克隆一种新的睾丸特异性蛋白丝氨酸/苏氨酸磷酸酶PPN 58A。

Cloning of a novel testis specific protein serine/threonine phosphatase, PPN 58A, from Drosophila melanogaster.

作者信息

Armstrong C G, Dombradi V, Mann D J, Cohen P T

机构信息

Department of Biochemistry, University of Dundee, UK.

出版信息

Biochim Biophys Acta. 1998 Aug 20;1399(2-3):234-8. doi: 10.1016/s0167-4781(98)00117-1.

DOI:10.1016/s0167-4781(98)00117-1

PMID:9765603

Abstract

A gene encoding a novel member of the PPP family of protein serine/threonine phosphatases, termed PPN 58A, was cloned from Drosophila melanogaster. The deduced amino acid sequence of PPN 58A exhibits 59-62% identity to D. melanogaster PP1 isoforms, 51% identity to D. melanogaster PPY 55A and < or = 40% identity to other members of the PPP family. The single copy gene PPN 58A maps to chromosome 2 locus 58A. Analysis of PPN 58A mRNA reveals that, like PPY 55A, PPN 58A is a testis specific enzyme.

摘要

从黑腹果蝇中克隆出一个编码蛋白丝氨酸/苏氨酸磷酸酶PPP家族新成员的基因，命名为PPN 58A。PPN 58A推导的氨基酸序列与黑腹果蝇PP1亚型有59 - 62%的同一性，与黑腹果蝇PPY 55A有51%的同一性，与PPP家族的其他成员同一性≤40%。单拷贝基因PPN 58A定位于2号染色体58A位点。对PPN 58A mRNA的分析表明，与PPY 55A一样，PPN 58A是一种睾丸特异性酶。