Dick T, Bahri S M, Chia W
Institute of Molecular and Cell Biology, National University of Singapore, Singapore.
Gene. 1997 Oct 15;199(1-2):139-43. doi: 10.1016/s0378-1119(97)00359-4.
We report the molecular cloning, chromosome mapping and developmental transcription pattern of a putative serine/threonine protein phosphatase 2C (PP2C), DPP2C1, from Drosophila melanogaster. The 6-kb transcript of this first Drosophila PP2C gene encodes a 1428-aa deduced protein. The DPP2C1 protein contains a approximately 330-aa PP2C-like catalytic domain flanked by extensive N- and C-terminal sequences showing no similarities to other PP2Cs. The dpp2c1 gene maps to 4E1-2 on the X chromosome, 1.5 kb upstream of the ddlc1 gene. Northern blot analyses showed that dpp2c1 transcription is developmentally regulated, accumulating maximally during early (0-6 h) and late (12-24 h) embryogensis. The presented molecular characterisation provides the basis for a genetic dissection of DPP2C1 function.
我们报道了来自黑腹果蝇的一个假定的丝氨酸/苏氨酸蛋白磷酸酶2C（PP2C），即DPP2C1的分子克隆、染色体定位及发育转录模式。这个首个果蝇PP2C基因的6kb转录本编码一个推导的含1428个氨基酸的蛋白质。DPP2C1蛋白包含一个约330个氨基酸的类PP2C催化结构域，两侧是广泛的N端和C端序列，这些序列与其他PP2C没有相似性。dpp2c1基因定位于X染色体上的4E1-2，在ddlc1基因上游1.5kb处。Northern印迹分析表明，dpp2c1转录受发育调控，在胚胎发育早期（0-6小时）和晚期（12-24小时）积累最多。所呈现的分子特征为DPP2C1功能的遗传剖析提供了基础。
