Shchepetkin I A
Institute of Oncology, Tomsk Research Center, Russian Academy of Medical Sciences.
Radiats Biol Radioecol. 1998 Jul-Aug;38(4):601-8.
The method of luminol-dependent chemiluminescence (CL) was used to study the influence of gamma- and fast electrons-irradiation on secretion of reactive oxygen species of neutrophils in enrich suspension and in presence of all blood components. In vitro gamma-irradiation (from 5 to 25 Gy) of the neutrophil suspension inhibited spontaneous CL and activated stimulated by phorbol-12-myristate-13-acetate (PMA) (100 nM) in the period 30-60 min after radiation. The time of maximum PMA-stimulated CL amplitude was decreased with the dose range from 2(5) to 25(30) Gy for gamma- and fast electrons-irradiation. The addition of radiosensitizer AK-2123 (0.05% or 2.2 mM) depressed the early PMA-stimulated CL response on gamma- and fast electrons-irradiation. The obtained results suggest that this effect is connected with influence of AK-2123 on ion canals of neutrophils.
采用鲁米诺依赖性化学发光（CL）方法，研究了γ射线和快电子辐照对富集悬浮液及全血成分存在时中性粒细胞活性氧分泌的影响。对中性粒细胞悬浮液进行体外γ射线辐照（5至25 Gy），在辐照后30至60分钟内，抑制了自发CL，并激活了由佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）（100 nM）刺激的CL。γ射线和快电子辐照剂量范围为2（5）至25（30）Gy时，PMA刺激的CL最大振幅时间缩短。添加放射增敏剂AK-2123（0.05%或2.2 mM）可抑制γ射线和快电子辐照后早期PMA刺激的CL反应。所得结果表明，这种效应与AK-2123对中性粒细胞离子通道的影响有关。
