利用分子工具鉴定表皮葡萄球菌的非典型菌株。

Identification of atypical strains of Staphylococcus epidermidis by use of molecular tools.

作者信息

Riou C, Meugnier H, Bes M, Brun Y, Fiedler F, Lasne Y, Etienne J, Freney J

机构信息

Département de Recherche en Bactériologie Médicale (UPRES EA 1655), Faculté de Médecine RTH Laennec, Lyon, France.

出版信息

Res Microbiol. 1997 Nov;148(8):715-24. doi: 10.1016/S0923-2508(99)80070-9.

DOI:10.1016/S0923-2508(99)80070-9

PMID:9765856

Abstract

Four atypical coagulase-negative staphylococcal (CNS) isolates from clinical sources were compared with Staphylococcus epidermidis strains by ribotyping. The ribotypes of the four strains shared close rDNA restriction profiles with those of the S. epidermidis strains used. The DNA sequence encoding 16S rRNA demonstrated 99.9% homology with S. epidermidis. S1 nuclease experiments showed that these atypical strains formed a homogeneous genomic group. DNA-DNA homologies between the S. epidermidis type strain CCM 2124 and the four CNS isolates ranged from 70 to 89%. The guanine-plus-cytosine content of the deoxyribonucleic acid of the four strains ranged from 31 to 32 mol%.

摘要

通过核糖体分型，将来自临床样本的4株非典型凝固酶阴性葡萄球菌（CNS）分离株与表皮葡萄球菌菌株进行了比较。这4株菌株的核糖体分型与所用表皮葡萄球菌菌株的核糖体DNA限制性图谱相近。编码16S rRNA的DNA序列与表皮葡萄球菌的同源性为99.9%。S1核酸酶实验表明，这些非典型菌株形成了一个同质基因组群。表皮葡萄球菌模式菌株CCM 2124与这4株CNS分离株之间的DNA-DNA同源性在70%至89%之间。这4株菌株的脱氧核糖核酸鸟嘌呤加胞嘧啶含量在31%至32%之间。