医院内粘质沙雷氏菌菌株的分型：用脉冲场凝胶电泳分析大分子限制性片段与生物分型、酯酶分型及核糖分型的比较

Typing of nosocomial strains of Serratia marcescens: comparison of pulsed-field gel electrophoresis of macrorestriction fragments with biotyping, esterase typing and ribotyping.

作者信息

Chetoui H, Delhalle E, Melin P, Struelens M J, De Ryck R, Osterrieth P, De Mol P

机构信息

Service de Microbiologie Médicale, Institut de Pathologie (B-23), Université de Liège, Belgium.

出版信息

Res Microbiol. 1998 Feb;149(2):137-43. doi: 10.1016/s0923-2508(98)80028-4.

DOI:10.1016/s0923-2508(98)80028-4

PMID:9766216

Abstract

Fifty nosocomial isolates of Serratia marcescens, collected in six Belgian hospitals between 1986 and 1990, were characterized by using pulsed-field gel electrophoresis (PFGE) with XbaI. The results were compared with those previously obtained by three other methods: biotyping, esterase electrophoresis typing and ribotyping with EcoRI and HindIII. Macrorestriction analysis (42 PFGE groups) and esterase typing (42 zymotypes) proved to be the most discriminating, followed by ribotyping (28 ribotypes) and biotyping (10 biochemical profiles). Biotyping would serve as a screen to identify isolates, due to its accessibility. Esterase typing provided a reliable tool to make subdivisions within biotypes because of congruence between biochemical groups and esterase patterns. Additional discrimination was still achieved by ribotyping and PFGE. It is concluded that the combined results of these four markers were useful for distinguishing all epidemic and sporadic isolates.

摘要

1986年至1990年间在比利时六家医院收集的50株医院内分离的粘质沙雷氏菌，采用XbaI脉冲场凝胶电泳（PFGE）进行特征分析。将结果与之前通过其他三种方法获得的结果进行比较：生物分型、酯酶电泳分型以及用EcoRI和HindIII进行核糖体分型。宏观限制性分析（42个PFGE组）和酯酶分型（42种酶型）被证明是最具鉴别力的，其次是核糖体分型（28种核糖体型）和生物分型（10种生化谱型）。由于生物分型易于操作，可作为鉴定分离株的筛选方法。由于生化组和酯酶模式之间的一致性，酯酶分型为在生物型内进行细分提供了可靠的工具。核糖体分型和PFGE仍能实现进一步的鉴别。得出的结论是，这四种标记的综合结果有助于区分所有流行和散发的分离株。