Fischer G, Holl A C, Volff J N, Vandewiele D, Decaris B, Leblond P
Laboratoire de Génétique et Microbiologie, Unité associée INRA 952, Faculté des Sciences, Université Henri Poincaré, Nancy 1, France.
Res Microbiol. 1998 Mar;149(3):203-10. doi: 10.1016/s0923-2508(98)80080-6.
From a cosmid clone of Streptomyces ambofaciens containing the dnaA and gyrAB genes, a 2.7-kb self-replicating DNA fragment containing the chromosome replication origin oriC was isolated. This cosmid was previously maped physically to a region near the middle of the 8-Mb linear chromosomal DNA. A pulsed-field gel electrophoresis time-course analysis revealed that sequences flanking oriC were overrepresented relative to the rest of the chromosomal DNA during rapid growth, indicating that this origin is active. In addition, the terminal regions of the chromosomal DNA showed a slight overrepresentation at the onset of stationary phase.
从含有dnaA和gyrAB基因的产二素链霉菌黏粒克隆中，分离出一个包含染色体复制起点oriC的2.7kb自我复制DNA片段。该黏粒先前已通过物理方法定位到8Mb线性染色体DNA中部附近的一个区域。脉冲场凝胶电泳时间进程分析表明，在快速生长期间，oriC侧翼序列相对于染色体DNA的其余部分过度富集，这表明该起点是活跃的。此外，染色体DNA的末端区域在稳定期开始时略有过度富集。
