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正常和无叶荔枝螺胚胎中RNA合成的电泳分析。

An electrophoretic analysis of RNA synthesis in normal and lobeless Ilyanassa embryo.

作者信息

Koser R B, Collier J R

出版信息

Differentiation. 1976 Mar 16;6(1):47-52. doi: 10.1111/j.1432-0436.1976.tb01467.x.

DOI:10.1111/j.1432-0436.1976.tb01467.x
PMID:976651
Abstract

RNA from pulse-labelled normal and lobeless Illyanassa embryos was analysed by electrophoresis on polyacrylamide gels. The proportional distribution of radioactivity among the RNAs made by these two types of embryos was compared statistically. It was shown that removal of the third polar lobe from the Ilyanassa egg at first cleavage did not produce any change in the proportion of several size classes of RNA transcribed during the first day od development. The post-gastrular lobeless embryo did show a significant deviation from the normal pattern of RNA synthesis, though it is not clear that this was a direct effect on transcription by the polar lobe cytoplasm.

摘要

对脉冲标记的正常和无叶状极体的伊利亚那萨胚胎的RNA进行聚丙烯酰胺凝胶电泳分析。对这两种类型胚胎产生的RNA中放射性的比例分布进行了统计学比较。结果表明,在第一次卵裂时从伊利亚那萨卵中去除第三极叶,在发育第一天转录的几种大小类别的RNA比例上没有产生任何变化。原肠胚后的无叶状极体胚胎确实显示出与正常RNA合成模式有显著偏差,尽管尚不清楚这是否是极叶细胞质对转录的直接影响。

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