Roos A, Schilder-Tol E J, Weening J J, Aten J
Department of Pathology, Academic Medical Center, University of Amsterdam, The Netherlands.
J Leukoc Biol. 1998 Oct;64(4):503-10. doi: 10.1002/jlb.64.4.503.
CD134 (OX40) is involved in T cell costimulation and T cell-dependent antibody production. We show strongly increased T cell expression of CD134 in a model of T helper 2-mediated systemic autoimmunity, induced by HgCl2. Regulation of CD134 expression on CD4+ T cells was further studied in vitro, identifying CD134 as an early marker of T cell activation. CD134 expression could be induced by interleukin-4, but not by interferon-gamma or tumor necrosis factor-alpha. Effects of interleukin-4 and of phorbol 12-myristate 13-acetate on CD134 expression could be blocked by the protein kinase inhibitor staurosporin. Combination of these stimuli with ionomycin resulted in a strongly synergistic increase of CD134 expression, which was blocked by the calcineurin-inhibitor cyclosporin A. The results demonstrate the involvement of two synergistically acting pathways in induction of CD134 expression. Furthermore, they suggest a role for interleukin-4 in induction of CD134 expression in vivo.
CD134（OX40）参与T细胞共刺激和T细胞依赖性抗体产生。我们发现在由氯化汞诱导的辅助性T细胞2介导的系统性自身免疫模型中，T细胞CD134的表达显著增加。在体外进一步研究了CD4 + T细胞上CD134表达的调节，确定CD134为T细胞活化的早期标志物。白细胞介素-4可诱导CD134表达，但干扰素-γ或肿瘤坏死因子-α不能诱导。蛋白激酶抑制剂星形孢菌素可阻断白细胞介素-4和佛波醇12-肉豆蔻酸酯13-乙酸酯对CD134表达的影响。这些刺激与离子霉素联合导致CD134表达强烈协同增加，而钙调神经磷酸酶抑制剂环孢素A可阻断这种增加。结果证明了两条协同作用途径参与CD134表达的诱导。此外，它们提示白细胞介素-4在体内CD134表达诱导中起作用。
