Pacheco Y M, Pérez-Camino M C, Cert A, Montero E, Ruiz-Gutiérrez V
Instituto de la Grasa (CSIC), Seville, Spain.
J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):127-32. doi: 10.1016/s0378-4347(98)00231-x.
The free diacylglycerols (DAGs) in adipose tissue are involved in the metabolism of stored lipids and hence are related to the supply of fatty acids for other tissues. This paper describes a simple, fast, and reproducible method for the identification and quantification of different molecular species of DAGs in human adipose tissue. The method comprised solid-phase extraction on a diol-bonded phase column combined with capillary GC analysis of silylated DAG derivatives on a polar phase (65% phenylmethylsilicone). Separation of the DAGs was achieved based on chain length, isomeric structure (1,2- and 1,3-DAGs), and degree of unsaturation. The main DAGs were 1,2-OO, 1,2-OP, 1,2-LO and 1,2-LP. The composition was corroborated by analysis of the component fatty acids of the DAGs, 18:1(n-9), 16:0, and 18:2(n-6) being the three major fatty acids obtained.
脂肪组织中的游离二酰基甘油（DAGs）参与储存脂质的代谢，因此与其他组织的脂肪酸供应有关。本文描述了一种用于鉴定和定量人脂肪组织中不同分子种类DAGs的简单、快速且可重复的方法。该方法包括在二醇键合相柱上进行固相萃取，并结合在极性相（65%苯基甲基硅氧烷）上对硅烷化DAG衍生物进行毛细管气相色谱分析。基于链长、异构体结构（1,2-和1,3-DAGs）以及不饱和度实现了DAGs的分离。主要的DAGs为1,2-OO、1,2-OP、1,2-LO和1,2-LP。通过对DAGs的组成脂肪酸进行分析证实了其组成，所获得的三种主要脂肪酸为18:1(n-9)、16:0和18:2(n-6)。
