Iglarz M, Baune B, Gantier J C, Hocquemiller R, Farinotti R
Service de Pharmacie Clinique et des Biomatériaux, G H X Bichat-Cl Bernard, Paris, France.
J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):335-40. doi: 10.1016/s0378-4347(98)00219-9.
A high-performance liquid chromatographic method was developed for the specific determination of 2-n-propylquinoline, a new anti-leishmaniasis drug, in plasma and liver homogenates of mice. 2-n-Propylquinoline was extracted with methyl-tert.-butyl ether with quinoline as internal standard. Separation was carried out using a Nucleosil C18 column. The mobile phase consisted of methanol-0.005 M ammonium acetate buffer (60:40) at pH 5.5 and 8 for plasma and liver homogenates, respectively. Detection was monitored at 233 nm. The method was validated and shown to be accurate and precise for plasma and liver homogenates. Extraction yield was 96% in plasma and 81% in liver homogenates. This method was used to determine the pharmacokinetic profile of 2-n-propylquinoline following oral administration to mice.
建立了一种高效液相色谱法，用于特异性测定新型抗利什曼病药物2-正丙基喹啉在小鼠血浆和肝脏匀浆中的含量。以喹啉为内标，用甲基叔丁基醚萃取2-正丙基喹啉。采用Nucleosil C18柱进行分离。流动相分别由甲醇-0.005M醋酸铵缓冲液（60:40）组成，血浆和肝脏匀浆的pH值分别为5.5和8。在233nm处进行检测。该方法经过验证，对血浆和肝脏匀浆具有准确性和精密度。血浆中的提取率为96%，肝脏匀浆中的提取率为81%。该方法用于测定小鼠口服2-正丙基喹啉后的药代动力学特征。
