使用高效液相色谱法检测恶性疟原虫疟疾患者在接受甲磺喹酮治疗后血浆和血细胞中的磺胺林浓度。

Sulfalene concentrations in plasma and blood cells of Plasmodium falciparum malaria cases after treatment with metakelfin using high-performance liquid chromatography.

作者信息

Dua V K, Sarin R, Gupta N C, Sharma V P

机构信息

Malaria Research Centre (Field Station), Health Centre, Sctor III, BHEL, Ranipur, Hardwar, India.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):390-4. doi: 10.1016/s0378-4347(98)00222-9.

DOI:10.1016/s0378-4347(98)00222-9

PMID:9766882

Abstract

A reversed-phase high-performance liquid chromatographic method using acetonitrile-methanol-1 M perchloric acid-water (25:9:0.8:95, v/v/v) at a flow-rate of 1.0 ml min(-1) on LiChrospher 100 RP 18 column (250 x 4 mm; 5 microm) with UV (254 nm) detection has been developed for the determination of sulfalene in plasma and blood cells after oral administration of the antimalarial drug metakelfin. Calibration curves were linear in the range 0.5-100 microg ml(-1). The limit of quantification was 50 ng ml(-1). Within-day and day-to-day coefficients of variation averaged 3.84 and 5.31%, respectively. Mean extraction recoveries of sulfalene from plasma and blood cells were 87.21 and 84.65%, respectively. Mean concentrations of sulfalene in plasma of P. falciparum cases on days 2, 7 and 15 were 44.58, 14.90 and 1.70 microg ml(-1), respectively; in blood cells concentrations of sulfalene were 7.77, 3.25 and 0.75 microg ml(-1), respectively, after oral treatment with two tablets (1000 mg) of metakelfin. Significant difference was recorded on day 2 for sulfalene concentration in blood cells of healthy and P. falciparum cases (t=9.49; P<0.001).

摘要

建立了一种反相高效液相色谱法，采用乙腈 - 甲醇 - 1 M高氯酸 - 水（25:9:0.8:95，v/v/v），流速为1.0 ml min⁻¹，在LiChrospher 100 RP 18柱（250×4 mm；5 µm）上，通过紫外（254 nm）检测来测定口服抗疟药美他氯喹后血浆和血细胞中的磺胺林。校准曲线在0.5 - 100 μg ml⁻¹范围内呈线性。定量限为50 ng ml⁻¹。日内和日间变异系数平均分别为3.84%和5.31%。磺胺林从血浆和血细胞中的平均提取回收率分别为87.21%和84.65%。恶性疟原虫病例在第2、7和15天血浆中磺胺林的平均浓度分别为44.58、14.90和1.70 μg ml⁻¹；口服两片（1000 mg）美他氯喹治疗后，血细胞中磺胺林的浓度分别为7.77、3.25和0.75 μg ml⁻¹。健康人和恶性疟原虫病例血细胞中磺胺林浓度在第2天有显著差异（t = 9.49；P < 0.001）。