Demel R A, Dorrepaal E, Ebskamp M J, Smeekens J C, de Kruijff B
Department Biochemistry of Membranes, Centre for Biomembranes and Lipid Enzymology, Utrecht University, Padualaan 8, NL-3584 CH Utrecht, Netherlands.
Biochim Biophys Acta. 1998 Oct 15;1375(1-2):36-42. doi: 10.1016/s0005-2736(98)00138-2.
Bacterial fructans with a high degree of polymerisation cause a very large increase in surface pressure of lipid monolayers at the air-water interface with a broad range of lipids, including phosphatidylethanolamine and several types of phosphatidylcholines. The surface active effect of fructans contrasts strongly with the maximal effects observed for trehalose, sucrose and glucose under comparable conditions (20 and 0.6 mN/m for fructans and the other sugars, respectively). The results demonstrate a profound and specific membrane interaction of the fructans which is probably very different from the effect of the smaller carbohydrates. The fructan concentrations used in this study are within the physiological range observed in fructan-accumulating plants. The suggested water-stress protective effect of fructans may be induced by membrane-fructan interaction which prevent lipid condensation and phase transitions to take place.
具有高度聚合度的细菌果聚糖会使脂质单分子层在空气-水界面的表面压力大幅增加，该脂质单分子层包含多种脂质，如磷脂酰乙醇胺和几种类型的磷脂酰胆碱。在可比条件下（果聚糖和其他糖类分别为20和0.6 mN/m），果聚糖的表面活性效应与海藻糖、蔗糖和葡萄糖所观察到的最大效应形成强烈对比。结果表明果聚糖与膜存在深刻且特定的相互作用，这可能与较小碳水化合物的作用有很大不同。本研究中使用的果聚糖浓度处于在积累果聚糖的植物中观察到的生理范围内。果聚糖所建议的水分胁迫保护作用可能是由膜-果聚糖相互作用诱导的，这种相互作用可防止脂质凝聚和相变发生。
