Liu Q X, Ueda H, Hirose S
Department of Developmental Genetics, National Institute of Genetics, and Department of Genetics, Graduate University for Advanced Studies, Mishima, Shizuoka-ken 411-8540, Japan.
Gene. 1998 Oct 5;220(1-2):55-9. doi: 10.1016/s0378-1119(98)00428-4.
MBF2 was first isolated from the silkworm Bombyx mori as a positive cofactor that activates transcription through its interaction with TFIIA. To identify conserved domain(s) within the MBF2 molecule, we isolated cDNAs encoding MBF2 homologues from other silkworms Bombyx mandarina and Samia cynthia. Bacterially expressed and purified MBF2 of B. mandarina and S. cynthia activated transcription in vitro. The predicted amino acid sequences of MBF2 from two Bombyx species share 97% homology. When we compared between B. mori and S. cynthia factors, the homology reduced to 50%. Four regions in MBF2 are conserved among these three species. Two of them are present in the middle region of MBF2 that is essential for the transcriptional activation.
MBF2最初是从家蚕中分离出来的一种正向辅助因子，它通过与TFIIA相互作用来激活转录。为了鉴定MBF2分子中的保守结构域，我们从其他蚕类野桑蚕和蓖麻蚕中分离出编码MBF2同源物的cDNA。在细菌中表达并纯化的野桑蚕和蓖麻蚕的MBF2在体外激活了转录。两种家蚕的MBF2预测氨基酸序列具有97%的同源性。当我们比较家蚕和蓖麻蚕的因子时，同源性降至50%。MBF2中的四个区域在这三个物种中是保守的。其中两个位于MBF2的中间区域，这对转录激活至关重要。
